Human GTF3C6 knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human GTF3C6 knockout HEK-293T cell lysate (AB263224)
Homozygous : 2 bp deletion in exon 1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein contributes to the transcription of small non-coding RNA genes such as 5S rRNA and tRNAs. GTF3C6 is a subunit of the TFIIIC complex and its function involves the recognition of specific promoter elements on DNA which is imperative for initiating transcription. By participating in the recognition and structuring of promoter regions GTF3C6 ensures proper transcription activation necessary for cell growth and metabolism.
Pathways
The engagement of GTF3C6 in RNA polymerase III transcription is significant. It operates within the RNA polymerase III transcription pathways interacting with TFIIIB and RNA polymerase III which are key components of the transcription machinery. GTF3C6 plays a role in the complex network of transcription regulation ensuring the timely transcription of essential RNAs that regulate protein synthesis and cell cycle progression.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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