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AB257462

Human GYS1 (Glycogen synthase 1) knockout HeLa cell lysate

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GYS1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.
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Western blot - Human GYS1 (Glycogen synthase 1) knockout HeLa cell lysate (AB257462)
  • WB

Lab

Western blot - Human GYS1 (Glycogen synthase 1) knockout HeLa cell lysate (AB257462)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : Glycogen synthase 1/GYS1 knockout HeLa cell lysate (20 μg)

Lane 3 : HEK-293 cell lysate (20 μg)

Lane 4 : Daudi cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab40810 observed at 80 kDa. Red - loading control ab8245 observed at 37 kDa.

ab40810 Recombinant Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] was shown to specifically react with Glycogen synthase 1/GYS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265388 (knockout cell lysate ab257462) was used. Wild-type and Glycogen synthase 1/GYS1 knockout samples were subjected to SDS-PAGE. ab40810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (<a href='/en-us/products/primary-antibodies/glycogen-synthase-1-gys1-antibody-ep817y-ab40810'>ab40810</a>) at 1/10000 dilution

Lane 1:

Wild-type HeLa lysate at 20 µg

Lane 2:

Glycogen synthase 1/GYS1 knockout HeLa lysate at 20 µg

Lane 2:

Western blot - Human GYS1 (Glycogen synthase 1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-gys1-glycogen-synthase-1-knockout-hela-cell-line-ab265388'>ab265388</a>)

Lane 3:

HEK-293 lysate at 20 µg

Lane 4:

Daudi lysate at 20 µg

Predicted band size: 84 kDa

Observed band size: 80 kDa

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Sanger Sequencing - Human GYS1 (Glycogen synthase 1) knockout HeLa cell lysate (AB257462)
  • Sanger seq

Unknown

Sanger Sequencing - Human GYS1 (Glycogen synthase 1) knockout HeLa cell lysate (AB257462)

Homozygous : 1 bp deletion in exon1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.

Disease

Adenocarcinoma

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Complementary Products

Primary Antibodies

AB40810

Anti-Glycogen synthase 1/GYS1 antibody [EP817Y]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (AB40810)

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Cell Lines & Lysates

AB261678

Human SLC2A1 (Glucose Transporter GLUT1) knockout A549 cell lysate

Western blot - Human SLC2A1 (Glucose Transporter GLUT1) knockout A549 cell lysate (AB261678)

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  • 0
Cell Lines & Lysates

AB258207

Human SRGN (Serglycin) knockout HeLa cell lysate

Sanger Sequencing - Human SRGN (Serglycin) knockout HeLa cell lysate (AB258207)

  • 0
  • 0
Cell Lines & Lysates

AB269670

Human SIRT1 knockout HEK-293 cell lysate

  • 0
  • 0
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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
GYS1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycogen synthase 1 (GYS1) also known as muscle glycogen synthase catalyzes the synthesis of glycogen from glucose. It adds glucose units from UDP-glucose to the non-reducing ends of glycogen chains facilitating glycogen elongation. The molecular mass of GYS1 is approximately 85 kDa. It is predominantly expressed in skeletal muscle tissue providing energy storage primarily in the form of glycogen. GYS1 is regulated through phosphorylation wherein it undergoes inactive to active conformational changes.
Biological function summary

This enzyme plays a critical role in the energy storage mechanism of muscle tissues. GYS1 functions as a part of the glycogen synthase complex interacting with various glycogen-associated proteins to modulate its activity. These interactions control the enzyme's affinity for its substrate and its susceptibility to regulatory signals like phosphorylation affecting glycogen synthesis efficiency. Through its activity GYS1 ensures a readily available reserve of glucose in muscle tissues for rapid energy production when required.

Pathways

Glycogen synthase 1 is a central component of the glycogen metabolism pathway. It acts alongside other enzymes such as glycogen phosphorylase which catalyzes glycogen breakdown. The balance between GYS1 and glycogen phosphorylase determines the rate of glycogen turnover within cells. GYS1 is further regulated by the insulin signaling pathway where insulin promotes glycogen synthesis by altering GYS1 activity alongside proteins like AKT influencing glucose homeostasis and energy management in tissues.

Dysregulation of GYS1 activity often associates with glycogen storage diseases notably Type 0 where deficiencies in glycogen storage lead to episodes of low blood sugar and reduced exercise capacity. Furthermore aberrations in GYS1 function contribute to insulin resistance and Type 2 diabetes conditions linked with impaired glucose metabolism. Altered GYS1 activity impacts proteins like glycogen phosphorylase affecting overall glycogen metabolism balance and contributing to metabolic disorders.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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