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AB263919

Human H1F0 knockout A-431 cell lysate

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H1-0 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included.

View Alternative Names

H1 histone family member 0, H1(0), H10_HUMAN, H1FV, H1f0, Histone H1'', Histone H1(0), Histone H1.0, Histone H10, Histone H5, MGC5241, N-terminally processed

4 Images
Western blot - Human H1F0 knockout A-431 cell lysate (AB263919)
  • WB

Lab

Western blot - Human H1F0 knockout A-431 cell lysate (AB263919)

Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate

Lane 2 : H1F0 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate

Lanes 1 - 2 : Merged signal (red and green). Green - ab125027 observed at 33 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

ab125027 was shown to react with H1F0 in wild-type A-431 cells in Western blot Loss of signal was observed when H1F0 knockout cell line ab262478 (knockout cell lysate ab263919) was used. Wild-type A-431 and H1F0 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab125027 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Histone H1.0 antibody [EPR6537] (<a href='/en-us/products/primary-antibodies/histone-h10-antibody-epr6537-ab125027'>ab125027</a>) at 1/1000 dilution

Lane 1:

Wild-type A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

H1F0 knockout A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human H1F0 knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-h1f0-knockout-a-431-cell-line-ab262478'>ab262478</a>)

Predicted band size: 21 kDa

Observed band size: 33 kDa

false

Western blot - Human H1F0 knockout A-431 cell lysate (AB263919)
  • WB

Lab

Western blot - Human H1F0 knockout A-431 cell lysate (AB263919)

Lane 1 : Wild-type A431 cell lysate

Lane 2 : H1F0 knockout A431 cell lysate

Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lanes 1 - 3 : Merged signal (red and green). Green - ab11080 observed at 30 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

ab11080 was shown to react with Histone H1 knockout cell line ab262478 (knockout cell lysate ab263919).0 in wild-type A-431 cells in Western blot with loss of signal observed in H1F0 knockout sample. Wild-type A-431 and H1F0 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab11080 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Histone H1.0 antibody [27] (<a href='/en-us/products/primary-antibodies/histone-h10-antibody-27-ab11080'>ab11080</a>) at 1/500 dilution

Lane 1:

Wild-type A431 cell lysate at 40 µg

Lane 2:

H1F0 knockout A431 cell lysate at 40 µg

Lane 2:

Western blot - Human H1F0 knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-h1f0-knockout-a-431-cell-line-ab262478'>ab262478</a>)

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 40 µg

Predicted band size: 21 kDa

Observed band size: 30 kDa

false

Western blot - Human H1F0 knockout A-431 cell lysate (AB263919)
  • WB

Lab

Western blot - Human H1F0 knockout A-431 cell lysate (AB263919)

Lane 1 : Wild-type A431 cell lysate

Lane 2 : H1F0 knockout A431 cell lysate

Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lanes 1 - 3 : Merged signal (red and green). Green - ab134914 observed at 30 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

ab134914 was shown to react with Histone H1.0 in wild-type A-431 cells in Western blot. Loss of signal was observed when H1F0 knockout cell line ab262478 (knockout cell lysate ab263919) was used. Wild-type and H1F0 A-431 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134914 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Histone H1.0 antibody [EPR6536] (<a href='/en-us/products/primary-antibodies/histone-h10-antibody-epr6536-ab134914'>ab134914</a>) at 1/1000 dilution

Lane 1:

Wild-type A431 cell lysate at 20 µg

Lane 2:

H1F0 knockout A431 cell lysate at 20 µg

Lane 2:

Western blot - Human H1F0 knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-h1f0-knockout-a-431-cell-line-ab262478'>ab262478</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 21 kDa

Observed band size: 30 kDa

false

Western blot - Human H1F0 knockout A-431 cell lysate (AB263919)
  • WB

Lab

Western blot - Human H1F0 knockout A-431 cell lysate (AB263919)

Lane 1 : Wild-type A431 cell lysate

Lane 2 : H1F0 knockout A431 cell lysate

Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lanes 1 - 3 : Merged signal (red and green). Green - ab11079 observed at 30 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

ab11079 was shown to react with Histone H1 knockout cell line ab262478 (knockout cell lysate ab263919).0 in wild-type A-431 cells in Western blot with loss of signal observed in H1F0 knockout sample. Wild-type A-431 and H1F0 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab11079 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Histone H1.0 antibody [34] (<a href='/en-us/products/primary-antibodies/histone-h10-antibody-34-ab11079'>ab11079</a>) at 1/500 dilution

Lane 1:

Wild-type A431 cell lysate at 40 µg

Lane 2:

H1F0 knockout A431 cell lysate at 40 µg

Lane 2:

Western blot - Human H1F0 knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-h1f0-knockout-a-431-cell-line-ab262478'>ab262478</a>)

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 40 µg

Predicted band size: 21 kDa

Observed band size: 30 kDa

false

Key facts

Cell type

A-431

Species or organism

Human

Tissue

Skin

Knockout validation

Next Generation Sequencing,Western blot

Disease

Epidermoid Carcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
H1-0
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB134914

Anti-Histone H1.0 antibody [EPR6536]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

  • 5
  • 1
Proteins & Peptides

AB198676

Recombinant Human Histone H1 protein

SDS-PAGE - Recombinant Human Histone H1 protein (AB198676)

  • 0
  • 0

Product promise

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