Human H2AFY (mH2A1) knockout HEK-293T cell lysate
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MACROH2A1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 2.
View Alternative Names
Core histone macro-H2A.1, H2A histone family member Y, H2A.y, H2A/y, H2AF12M, H2AFJ, H2AY_HUMAN, H2afy, Histone H2A.Y, Histone macroH2A1, Histone macroH2A1.1, Histone macroH2A1.2, MACROH2A1.1, MacroH2A1.2, Macroh2a1, Medulloblastoma antigen MU-MB-50.205, mH2A1, mH2a
- WB
Lab
Western blot - Human H2AFY (mH2A1) knockout HEK-293T cell lysate (AB257463)
Lane 1 : Wild-type HEK-293T cell lysate (20µg)
Lane 2 : H2AFY knockout HEK-293T cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab183041 observed at 40 kDa. Red - loading control, ab7291 observed at 50 kDa.
ab183041 Anti-mH2A1 antibody [EPR9359(2)] was shown to specifically react with mH2A1 in wild-type HEK-293T cells in western blot. The band observed in the knockout cell line ab266241 (knockout cell lysate ab257463) lane below 40kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and mH2A1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab183041 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-mH2A1 antibody [EPR9359(2)] (<a href='/en-us/products/primary-antibodies/mh2a1-antibody-epr93592-ab183041'>ab183041</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
H2AFY knockout HEK-293T cell lysate at 20 µg
Predicted band size: 40 kDa
Observed band size: 40 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human H2AFY (mH2A1) knockout HEK-293T cell lysate (AB257463)
Homozygous : 13 bp deletion in exon 2
Reactivity data
Product details
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MH2A1 plays significant roles in chromatin remodeling and gene expression regulation. It is part of the nucleosome replacing canonical histone H2A and influencing the accessibility of chromatin to transcriptional machinery. Through this function mH2A1 can repress or activate gene expression depending on cellular context and interacting partners. Its incorporation into chromatin is associated with the silencing of certain genomic regions such as inactive X chromosome in females with potential implications for epigenetic modifications and cellular differentiation.
Pathways
MH2A1 is notably involved in pathways regulating chromatin organization and cell differentiation. It plays a role in the Polycomb Repressive Complex 2 (PRC2) pathway affecting histone methylation and gene silencing. Furthermore mH2A1 interacts with proteins like EZH2 in PRC2 which carry out methylation at histone H3 on lysine 27. These interactions highlight mH2A1's involvement in maintaining repressive chromatin states and controlling gene expression patterns important for normal cell function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Immunocytochemistry/ Immunofluorescence - Anti-mH2A1 antibody [EPR9359(2)] (AB183041)](https://content.abcam.com/products/images/mh2a1-antibody-epr93592-ab183041--immunocytochemistry-immunofluorescence-img126197.jpg)
Cell Lines & Lysates
AB266241
Human H2AFY (mH2A1) knockout HEK-293T cell line
Advanced Validation
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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