Human HCFC1R1 (Host cell factor C1 regulator 1) knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human HCFC1R1 (Host cell factor C1 regulator 1) knockout HEK-293T cell lysate (AB258892)
Allele-2 : Insertion of the selection cassette in exon 4
- Sanger seq
Unknown
Sanger Sequencing - Human HCFC1R1 (Host cell factor C1 regulator 1) knockout HEK-293T cell lysate (AB258892)
Allele-1 : 29 bp deletion in exon 4
Complementary Products
Cell Lines & Lysates
AB266341
Human HCFC1R1 (Host cell factor C1 regulator 1) knockout HEK-293T cell line
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Primary Antibodies
AB289975
Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant
![Immunocytochemistry/ Immunofluorescence - Anti-HCF-1 / Host Cell Factor C1 antibody [EPR25290-18] (AB289975)](https://content.abcam.com/products/images/hcf-1-host-cell-factor-c1-antibody-epr25290-18-ab289975--immunocytochemistry-immunofluorescence-img166652.jpg)
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Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HCFC1R1 functions by interacting with multiple partners in a dynamic multiprotein complex. It associates with Host Cell Factor C1 (HCFC1) as a core component of the complex contributing to the regulation of cell cycle progression and gene expression. The protein is also involved in histone modification processes which influence the epigenetic landscape of the cell. Additionally HCFC1R1 plays roles in DNA repair mechanisms and cellular response to damage accentuating its function in maintaining cellular integrity.
Pathways
HCFC1R1 integrates into essential signal transduction pathways notably the cell cycle control and DNA repair pathways. It associates with proteins such as Histone Deacetylase 1 (HDAC1) participating in chromatin remodeling which subsequently impacts cell cycle progression. HCFC1R1's role in the cell cycle is tightly linked with its regulatory function on the transcriptional activity of E2F transcription factors important components in the control of cell proliferation.
Quality control
STR analysis
D7S820, D5S818, TH01, D16S539, TPOX, CSF1PO, D13S317
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com