Human HDAC2 knockout HEK-293T cell lysate
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Human HDAC2 knockout HEK-293T cell lysate suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
View Alternative Names
D10Wsu179e, HD 2, HDAC2_HUMAN, Histone deacetylase 2, Histone deacetylase 2 (HD2), OTTHUMP00000017046, OTTHUMP00000227077, OTTHUMP00000227078, RPD 3, YAF1, YY1 associated factor 1, YY1 transcription factor binding protein, Yy1bp, transcriptional regulator homolog RPD3
- WB
Lab
Western blot - Human HDAC2 knockout HEK-293T cell lysate (AB256938)
Lane 1 : Wild-type HEK-293T cell lysate (20µg)
Lane 2 : HDAC2 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab124974 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.
ab124974 Rabbit monoclonal [EPR5001] to HDAC2 - ChIP Grade was shown to specifically react with HDAC2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266589 (knockout cell lysate ab256938) was used. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab124974 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-HDAC2 antibody [EPR5001] - ChIP Grade (<a href='/en-us/products/primary-antibodies/hdac2-antibody-epr5001-chip-grade-ab124974'>ab124974</a>) at 1/10000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
HDAC2 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human HDAC2 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hdac2-knockout-hek-293t-cell-line-ab266589'>ab266589</a>)
Predicted band size: 44 kDa,55 kDa
Observed band size: 45 kDa,47 kDa,55 kDa
false
- WB
Lab
Western blot - Human HDAC2 knockout HEK-293T cell lysate (AB256938)
Lane 1 : Wild-type HEK-293T cell lysate (20µg)
Lane 2 : HDAC2 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab219053 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.
ab219053 Rabbit monoclonal [EPR20117] to HDAC2 was shown to specifically react with HDAC2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266589 (knockout cell lysate ab256938) was used. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab219053 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 μg/ml and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-HDAC2 antibody [EPR20117] (<a href='/en-us/products/primary-antibodies/hdac2-antibody-epr20117-ab219053'>ab219053</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
HDAC2 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human HDAC2 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hdac2-knockout-hek-293t-cell-line-ab266589'>ab266589</a>)
Predicted band size: 55 kDa
Observed band size: 55 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human HDAC2 knockout HEK-293T cell lysate (AB256938)
Allele-1 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human HDAC2 knockout HEK-293T cell lysate (AB256938)
Allele-2 : 41 bp insertion in exon2
Reactivity data
Product details
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HDAC2 is involved in cell cycle regulation and differentiation. It is part of the co-repressor complex and interacts with other proteins such as Sin3 and NuRD to repress transcription. These interactions are vital for maintaining normal cellular functions and ensuring proper response to external signals. This protein is also connected to HEK293T cells which are a common model in scientific studies due to their robust expression of proteins like HDAC2.
Pathways
HDAC2 participates in the regulation of transcriptional activity and cellular stress response. It is an important player in the histone modification pathway interacting with proteins such as HDAC1 and transcriptional regulators like REST. HDAC2 is also linked with the Notch signaling pathway which is essential for cell fate decisions. These pathways highlight its role in both maintaining cellular homeostasis and adapting to changes in the cellular environment.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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