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HDAC2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon2 and 1 bp insertion in exon2.

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Images

Western blot - Human HDAC2 knockout HEK-293T cell lysate (AB256939), expandable thumbnail
  • Western blot - Human HDAC2 knockout HEK-293T cell lysate (AB256939), expandable thumbnail
  • Sanger Sequencing - Human HDAC2 knockout HEK-293T cell lysate (AB256939), expandable thumbnail
  • Sanger Sequencing - Human HDAC2 knockout HEK-293T cell lysate (AB256939), expandable thumbnail

Key facts

Cell type
HEK-293T
Species or organism
Human
Tissue
Kidney
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon2 and 1 bp insertion in exon2.

Alternative names

D10Wsu179e, HD 2, HDAC2_HUMAN, Histone deacetylase 2, Histone deacetylase 2 (HD2), OTTHUMP00000017046, OTTHUMP00000227077, OTTHUMP00000227078, RPD 3, YAF1, YY1 associated factor 1, YY1 transcription factor binding protein, Yy1bp, transcriptional regulator homolog RPD3

What's included?

1 Kit
Components
Human HDAC2 knockout HEK293T cell lysate
1 x 100 µg
Human wild-type HEK293T cell lysate
1 x 100 µg

Recommended products

HDAC2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon2 and 1 bp insertion in exon2.

Key facts

Cell type
HEK-293T
Mutation description
Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon2 and 1 bp insertion in exon2.
Concentration
Loading...

Properties

Gene name
HDAC2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

HDAC2 or Histone Deacetylase 2 is an important enzyme that plays a role in chromatin remodeling and gene expression regulation. It removes acetyl groups from histone tails allowing chromatin to condense and silencing gene expression. HDAC2 has a molecular weight of approximately 55 kDa. It is expressed in various tissues including brain heart and skeletal muscles indicating its importance in different physiological areas. HDAC2 proteins are often studied using methods like HDAC2 ELISA and HDAC2 anticuero in research settings.

Biological function summary

HDAC2 is involved in cell cycle regulation and differentiation. It is part of the co-repressor complex and interacts with other proteins such as Sin3 and NuRD to repress transcription. These interactions are vital for maintaining normal cellular functions and ensuring proper response to external signals. This protein is also connected to HEK293T cells which are a common model in scientific studies due to their robust expression of proteins like HDAC2.

Pathways

HDAC2 participates in the regulation of transcriptional activity and cellular stress response. It is an important player in the histone modification pathway interacting with proteins such as HDAC1 and transcriptional regulators like REST. HDAC2 is also linked with the Notch signaling pathway which is essential for cell fate decisions. These pathways highlight its role in both maintaining cellular homeostasis and adapting to changes in the cellular environment.

Associated diseases and disorders

HDAC2 has a significant connection to neurodegenerative diseases and various cancers. Alzheimer's disease for instance shows altered expression levels of HDAC2 affecting synaptic plasticity and memory formation. In cancer HDAC2 interacts with oncogenic proteins such as p53 influencing tumor progression and metastasis. Monitoring HDAC2 levels and activity could provide insights into disease mechanisms and potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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