Human HELLS (SMARCA6) knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human HELLS (SMARCA6) knockout HeLa cell lysate (AB258893)
Allele-1 : 1 bp insertion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human HELLS (SMARCA6) knockout HeLa cell lysate (AB258893)
Allele-2 : Insertion of the selection cassette in exon 2
Complementary Products
Primary Antibodies
AB300040
Anti-SMARCA6 antibody [EPR25295-62]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant
![Immunocytochemistry/ Immunofluorescence - Anti-SMARCA6 antibody [EPR25295-62] (AB300040)](https://content.abcam.com/products/images/smarca6-antibody-epr25295-62-ab300040--immunocytochemistry-immunofluorescence-img170236.jpg)
- 0
- 0
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SMARCA6 influences gene expression by modifying the chromatin structure which allows transcription factors to access DNA. It is a part of the SWI/SNF chromatin remodeling complex critical for regulating a wide range of genes involved in development and cell cycle. Through the remodeling of chromatin SMARCA6 controls access to specific genomic regions and modulates transcriptional activation or repression therefore influencing cellular differentiation and proliferation.
Pathways
SMARCA6 participates in pathways involved in chromatin organization and gene regulation. It engages in the Wnt signaling pathway interacting with proteins such as BAF155 and BAF170 to modulate transcriptional outcomes. SMARCA6's activity in these pathways is essential for cellular processes impacting how cells respond to growth signals and maintain homeostasis.
Quality control
STR analysis
D7S820, D5S818, TH01, D16S539, TPOX, CSF1PO, D13S317
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com