Human HELZ2 knockout A549 cell lysate
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HELZ2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon9 and 1 bp insertion in exon9.
View Alternative Names
ATP-dependent helicase PRIC285, KIAA1769, PDIP1, PPAR-alpha-interacting complex protein 285, PPAR-gamma DBD-interacting protein 1, PPAR-gamma DNA-binding domain-interacting protein 1, PR285_HUMAN, PRIC285, PRPF8, Peroxisomal proliferator-activated receptor A-interacting complex 285 kDa protein
- Sanger seq
Unknown
Sanger Sequencing - Human HELZ2 knockout A549 cell lysate (AB258896)
Allele-1 : 1 bp deletion in exon9
- Sanger seq
Unknown
Sanger Sequencing - Human HELZ2 knockout A549 cell lysate (AB258896)
Allele-2 : 1 bp insertion in exon9
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HELZ2 functions in gene expression regulation by interacting with nuclear receptors and transcription factors. It acts within a protein complex influencing transcriptional regulation through chromatin remodeling. By modulating key transcriptional activities HELZ2 affects numerous genetic pathways and signal transductions contributing to fundamental cellular functions including development and differentiation. Knockout studies of HELZ2 dbd or domain-binding disruption suggest significant involvement in cellular homeostasis.
Pathways
This protein plays significant roles in lipid metabolism and the insulin signaling pathway. HELZ2 has connections to related proteins such as PPAR and SREBPs involved in regulating lipid homeostasis and metabolic balance. Through these pathways HELZ2 navigates the intricate regulatory network that maintains metabolic equilibrium exhibiting a profound influence on cellular metabolic processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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