Human HENMT1 knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
HENMT1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 3.
View Alternative Names
C1orf59, HEN1 methyltransferase homolog 1, HEN1 methyltransferase homolog 1 (Arabidopsis), HENMT_HUMAN, RP11-256E16.2, Small RNA 2' O methyltransferase, Small RNA 2''-O-methyltransferase
- Sanger seq
Unknown
Sanger Sequencing - Human HENMT1 knockout HeLa cell lysate (AB258897)
Homozygous : Insertion of the selection cassette in exon 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The enzyme HENMT1 participates in piRNA biogenesis. These piRNAs are part of a larger ribonucleoprotein complex that includes Piwi proteins which are essential for transposon silencing in the germline. This complex ensures the epigenetic and post-transcriptional silencing of transposable elements consequently protecting genomic integrity. HENMT1's activity is fundamental for safeguarding the genome from potential aberrations caused by active transposons.
Pathways
HENMT1 plays a significant part in the piRNA pathway which is important for gene silencing in the germline. Through this pathway it contributes to the protection of the genome against transposable elements by working alongside proteins like Piwi. The RNA interference (RNAi) pathway also involves HENMT1 although indirectly as RNAi and piRNA pathways share some mechanistic similarities in their mode of silencing unwanted genetic elements.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com