Human HERC5 knockout A549 cell lysate
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HERC5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 20 bp deletion in exon1.
View Alternative Names
CEB1, CEBP1, Cyclin-E-binding protein 1, E3 ISG15--protein ligase HERC5, HECT E3 ubiquitin ligase, HECT domain and RCC1-like domain-containing protein 5, HERC5_HUMAN, Hect domain and RLD 5
- Sanger seq
Unknown
Sanger Sequencing - Human HERC5 knockout A549 cell lysate (AB258898)
Allele-1 : 20 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human HERC5 knockout A549 cell lysate (AB258898)
Allele-2 : 1 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The HERC5 protein contributes to innate immune response by modifying proteins involved in antiviral defense. It adds ISG15 to newly synthesized proteins marking them for rapid degradation or altering their activities. HERC5 operates as a solitary protein rather than forming part of a larger complex. Through its ISGylation activity HERC5 plays a significant role in enhancing the antiviral state of cells inhibiting viral replication and modulating cellular processes during stress responses.
Pathways
HERC5 actively integrates into the antiviral defense mechanism and ubiquitin-proteasome pathway. It associates with pathways related to protein modification and immune signaling significantly affecting how cells respond to viral infections. Proteins like ISG15 and ubiquitin-conjugating enzymes interact with HERC5 in these pathways highlighting its importance in maintaining cellular homeostasis and protecting against pathogens.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Primary Antibodies
AB26721
Anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade
BOND RX™ Validated
- 4
- 12
![Western blot - Anti-BBS1 antibody [EPR7906] (AB166613)](https://content.abcam.com/products/images/bbs1-antibody-epr7906-ab166613--western-blot-img101196.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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