Human HINT1 knockout HeLa cell lysate
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(0 Publication)
- WB
Lab
Western blot - Human HINT1 knockout HeLa cell lysate (AB257465)
Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : HINT1 knockout HeLa cell lysate (20 ug)
Lane 3 : Jurkat cell lysate (20 ug)
ab124912 was shown to specifically react with HINT1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265776 (knockout cell lysate ab257465) was used. Wild-type and HINT1 knockout samples were subjected to SDS-PAGE. ab124912 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-HINT1 antibody [EPR5108] (<a href='/en-us/products/primary-antibodies/hint1-antibody-epr5108-ab124912'>ab124912</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human HINT1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-hint1-knockout-hela-cell-line-ab265776'>ab265776</a>)
Lane 2:
HINT1 knockout HeLa cell lysate at 20 µg
Lane 3:
Jurkat cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human HINT1 knockout HeLa cell lysate (AB257465)
Homozygous : 1 bp deletion in exon 1
Complementary Products
Primary Antibodies
AB240007
Anti-HINT1 antibody [EPR5108] - BSA and Azide free
RabMAb|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HINT1 antibody [EPR5108] - BSA and Azide free (AB240007)](https://content.abcam.com/products/images/hint1-antibody-epr5108-bsa-and-azide-free-ab240007--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102341.jpg)
- 0
- 0
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Within cellular processes HINT1 plays a role as a transcriptional regulator especially influencing the p53 pathway and those involved in stress responses. It forms part of a complex with other proteins guiding cellular responses to DNA damage. This activity strongly suggests HINT1's role in maintaining genomic stability and influencing cell cycle checkpoints.
Pathways
Several important signaling cascades involve HINT1 such as p53 and MAPK pathways. In the context of the p53 pathway HINT1 enhances tumor suppression activities by regulating transcription and apoptosis processes. Through the MAPK pathway it influences cell proliferation and apoptosis. Other proteins like p53 itself and c-Myc closely interact with HINT1 within these signaling pathways coordinating responses to cellular stress and growth signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com