HINT2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 1 and 1 bp insertion in exon 1 and 2 bp deletion in exon 1.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 1 and 1 bp insertion in exon 1 and 2 bp deletion in exon 1.
Alternative names
1190005L05Rik, HINT2_HUMAN, HIT-17, HIT-17kDa, Histidine triad nucleotide-binding protein 2, PKCI-1-related HIT protein, Protein kinase C inhibitor 2, mitochondrial
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HINT2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 1 and 1 bp insertion in exon 1 and 2 bp deletion in exon 1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 1 and 1 bp insertion in exon 1 and 2 bp deletion in exon 1.
- Concentration
Properties
- Gene name
- HINT2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HINT2 also known as histidine triad nucleotide-binding protein 2 is a mitochondrial protein weighing approximately 17 kDa. It involves in catalyzing the hydrolysis of nucleotide derivatives specifically adenosine 5'-monophosphoramidate substrates. You would usually find HINT2 in the liver kidney and heart where it likely plays roles in cellular metabolism and energy homeostasis.
- Biological function summary
This protein influences several cellular processes including apoptosis and mitochondrial function regulation. HINT2 is not part of a larger protein complex but works independently to ensure proper mitochondrial activity. Through these actions HINT2 maintains cellular homeostasis which is important for healthy energy production and utilization within the cell.
- Pathways
This protein interacts with key signaling pathways such as the mitochondrial apoptosis pathway. This pathway usually relates to programmed cell death and links closely with proteins like cytochrome c and caspases. By influencing this pathway HINT2 plays a part in determining cell fate and contributes to the balance between cell survival and death.
- Associated diseases and disorders
Research links HINT2 to metabolic syndromes and liver diseases. Alterations in this protein may affect how cells manage energy which could lead to or exacerbate conditions like non-alcoholic fatty liver disease. The relationship of HINT2 with transporter proteins such as carnitine palmitoyltransferase 1 suggests it might influence fatty acid metabolism providing insights into how energy imbalances can trigger or worsen metabolic issues.
Product promise
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Terms & Conditions.
3 product images
Sanger Sequencing - Human HINT2 knockout HEK-293T cell lysate (ab258902)
Allele-2: 2 bp deletion in exon 1
Sanger Sequencing - Human HINT2 knockout HEK-293T cell lysate (ab258902)
Allele-3: 1 bp insertion in exon 1
Sanger Sequencing - Human HINT2 knockout HEK-293T cell lysate (ab258902)
Allele-1: 17 bp deletion in exon 1
Downloads
Product protocols
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