Human HIPK3 (FIST) knockout HeLa cell lysate
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HIPK3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
View Alternative Names
ANPK, Androgen receptor-interacting nuclear protein kinase, DYRK6, FIST3, Fas-interacting serine/threonine-protein kinase, HIPK3_HUMAN, Homeodomain-interacting protein kinase 3, Homolog of protein kinase YAK1, PKY, RP24 297H17.5, YAK1
- Sanger seq
Unknown
Sanger Sequencing - Human HIPK3 (FIST) knockout HeLa cell lysate (AB258903)
Homozygous : 1 bp insertion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FIST contributes to cellular processes by regulating the transport of specific substrates such as ions and small molecules critical for cellular homeostasis and energy balance. This protein is often part of a larger transporter complex allowing it to interact with other proteins to modulate its transport activities efficiently. Its role becomes more significant in squamous cell carcinoma where altered transport activities could contribute to the development and progression of the cancer.
Pathways
FIST has significant involvement in the cellular energy metabolism and signaling pathways. One key pathway is the ion transport pathway where FIST interacts with several channel proteins to maintain ionic concentration and cellular excitability. Moreover it associates with ATP-binding cassette (ABC) transporters participating in the regulation of multidrug resistance which provides cancer cells with a mechanism to survive chemotherapeutic treatments.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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