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AB261682

Human HIST1H1C (Histone H1.2) knockout A549 cell lysate

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H1-2 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 8 bp deletion Frameshift = 99%.

View Alternative Names

H1 histone family member 2, H1.a, H12_HUMAN, H1F2, H1s-1, HIST1H1C, Histone 1 H1c, Histone H1.2, Histone H1c, Histone H1d, Histone H1s-1, Histone cluster 1 H1c, MGC3992

2 Images
Western blot - Human HIST1H1C (Histone H1.2) knockout A549 cell lysate (AB261682)
  • WB

Lab

Western blot - Human HIST1H1C (Histone H1.2) knockout A549 cell lysate (AB261682)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate

Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 3 : HIST1H1C knockout A549 (Human lung carcinoma cell line) whole cell lysate

Lanes 1 - 3 : Merged signal (red and green). Green - ab4086 observed at 30 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab4086 was shown to recognize HIST1H1C in wild-type A549 cells as signal was lost at the expected MW in HeLa knockout cell line ab261873 (knockout cell lysate ab261682). Additional cross-reactive bands were observed in the wild-type and knockout samples. Wild-type and HeLa knockout samples were subjected to SDS-PAGE. ab4086 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Histone H1.2 antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h12-antibody-chip-grade-ab4086'>ab4086</a>) at 1/500 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg/mL

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg/mL

Lane 2:

Western blot - Human HIST1H1C (Histone H1.2) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-hist1h1c-histone-h12-knockout-a549-cell-line-ab261873'>ab261873</a>)

Lane 3:

HIST1H1C knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 21 kDa

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Next Generation Sequencing - Human HIST1H1C (Histone H1.2) knockout A549 cell lysate (AB261682)
  • NGS

Lab

Next Generation Sequencing - Human HIST1H1C (Histone H1.2) knockout A549 cell lysate (AB261682)

X = 8 bp deletion

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9 X = 8 bp deletion Frameshift = 99%

Disease

Carcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
H1-2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Product promise

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