Human HIST1H1C (Histone H1.2) knockout HeLa cell lysate available now.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 22 bp deletion in exon 1 and 2 bp insertion in exon 1.
Alternative names
H1 histone family member 2, H1.a, H12_HUMAN, H1F2, H1s-1, HIST1H1C, Histone 1 H1c, Histone H1.2, Histone H1c, Histone H1d, Histone H1s-1, Histone cluster 1 H1c, MGC3992
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Human HIST1H1C (Histone H1.2) knockout HeLa cell lysate available now.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 22 bp deletion in exon 1 and 2 bp insertion in exon 1.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Western blot - Human HIST1H1C (Histone H1.2) knockout HeLa cell lysate (ab257218)
Lane 1: Wild-type HeLa cell lysate 20 μg
Lane 2: HIST1H1C knockout HeLa cell lysate 20 μg
False colour image of Western blot: Anti-Histone H1.2 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Histone H1.2 antibody ab17677 was shown to bind specifically to Histone H1.2. A band was observed at 32 kDa in wild-type HeLa cell lysates with no signal observed at this size in HIST1H1C knockout cell line Human HIST1H1C (Histone H1.2) knockout HeLa cell line ab261794 (knockout cell lysate ab257218). To generate this image, wild-type and HIST1H1C knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.All lanes: Western blot - Anti-Histone H1.2 antibody (Anti-Histone H1.2 antibody ab17677) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: HIST1H1C knockout HeLa cell lysate at 20 µg
SecondaryLanes 1 - 2: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Lanes 1 - 2: Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 32 kDa, 50 kDa
Western blot - Human HIST1H1C (Histone H1.2) knockout HeLa cell lysate (ab257218)
Lane 1: Wild-type HeLa cell lysate 20 μg
Lane 2: HIST1H1C knockout HeLa cell lysate 20 μg
False colour image of Western blot: Anti-Histone H1.2 antibody - ChIP Grade staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Histone H1.2 antibody - ChIP Grade ab4086 was shown to bind specifically to Histone H1.2. A band was observed at 37 kDa in wild-type HeLa cell lysates with no signal observed at this size in HIST1H1C knockout cell line Human HIST1H1C (Histone H1.2) knockout HeLa cell line ab261794 (knockout cell lysate ab257218). To generate this image, wild-type and HIST1H1C knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.All lanes: Western blot - Anti-Histone H1.2 antibody - ChIP Grade (Anti-Histone H1.2 antibody - ChIP Grade ab4086) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: HIST1H1C knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human HIST1H1C (Histone H1.2) knockout HeLa cell line (Human HIST1H1C (Histone H1.2) knockout HeLa cell line ab261794)
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 37 kDa
Sanger Sequencing - Human HIST1H1C (Histone H1.2) knockout HeLa cell lysate (ab257218)
Allele-1: 22 bp deletion in exon 1
Sanger Sequencing - Human HIST1H1C (Histone H1.2) knockout HeLa cell lysate (ab257218)
Allele-2: 2 bp insertion in exon 1
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