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AB275250

Human HK2 (Hexokinase II) knockout HCT116 cell lysate

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HK2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

View Alternative Names

DKFZp686M1669, HK 2, HK II, HXK2_HUMAN, Hexokinase 2 muscle, Hexokinase type II, Hexokinase-2, Muscle form hexokinase

2 Images
Western blot - Human HK2 (Hexokinase II) knockout HCT116 cell lysate (AB275250)
  • WB

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Western blot - Human HK2 (Hexokinase II) knockout HCT116 cell lysate (AB275250)

Lane 1 : Wild-type HCT116 cell lysate 20 ug
Lane 2 : HK2 knockout HCT116 cell lysate 20 ug
Lane 3 : HeLa cell lysate 20 ug
Lane 4 : SH-SY5Y cell lysate 20 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab209847 observed at 105 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab209847 was shown to react with Anti-Hexokinase II in wild-type HCT 116 cells in western blot with loss of signal observed in HK2 knockout cell line ab273721 (HK2 knockout cell lysate ab275250). HCT 116 wild-type and HK2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab209847 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Hexokinase II antibody [EPR20839] (<a href='/en-us/products/primary-antibodies/hexokinase-ii-antibody-epr20839-ab209847'>ab209847</a>) at 1/1000 dilution

Lane 1:

Wild-type HCT116 cell lysate at 20 µg

Lane 2:

HK2 knockout HCT116 cell lysate at 20 µg

Lane 2:

Western blot - Human HK2 (Hexokinase II) knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-hk2-hexokinase-ii-knockout-hct116-cell-line-ab273721'>ab273721</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 102 kDa

Observed band size: 105 kDa

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Sanger Sequencing - Human HK2 (Hexokinase II) knockout HCT116 cell lysate (AB275250)
  • Sanger seq

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Sanger Sequencing - Human HK2 (Hexokinase II) knockout HCT116 cell lysate (AB275250)

Allele-1 : 1 bp insertion in exon 2.

Key facts

Cell type

HCT116

Species or organism

Human

Tissue

Colon

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2

Disease

Carcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
HK2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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