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AB281353

Human HK2 (Hexokinase II) knockout HEK-293 cell lysate

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HK2 KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 5 bp deletion Frameshift: 99%.

View Alternative Names

DKFZp686M1669, HK 2, HK II, HXK2_HUMAN, Hexokinase 2 muscle, Hexokinase type II, Hexokinase-2, Muscle form hexokinase

2 Images
Western blot - Human HK2 (Hexokinase II) knockout HEK-293 cell lysate (AB281353)
  • WB

Lab

Western blot - Human HK2 (Hexokinase II) knockout HEK-293 cell lysate (AB281353)

Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug
Lane 2 : HK2 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate 20 ug
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug
Lane 4 : A549 (Human lung carcinoma cell line) whole cell lysate 20 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab209847 observed at 110 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab209847 was shown to bind specifically to HK2. A band was observed at 110 kDa in wild-type cell lysates with no signal at this size in HK2 knockout cell line ab269485 (knockout cell lysate ab281353). Wild-type and HK2 knockout HEK-293 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab209847 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Hexokinase II antibody [EPR20839] (<a href='/en-us/products/primary-antibodies/hexokinase-ii-antibody-epr20839-ab209847'>ab209847</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

HK2 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human HK2 (Hexokinase II) knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-hk2-hexokinase-ii-knockout-hek-293-cell-line-ab269485'>ab269485</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 102 kDa

Observed band size: 110 kDa

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Next Generation Sequencing - Human HK2 (Hexokinase II) knockout HEK-293 cell lysate (AB281353)
  • NGS

Supplier Data

Next Generation Sequencing - Human HK2 (Hexokinase II) knockout HEK-293 cell lysate (AB281353)

Knockout achieved by CRISPR/Cas9; X = 5 bp deletion; Frameshift : 99%

Key facts

Cell type

HEK-293

Species or organism

Human

Tissue

Kidney

Knockout validation

Next Generation Sequencing

Mutation description

Knockout achieved by CRISPR/Cas9 X = 5 bp deletion Frameshift: 99%

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.</p>" } } }
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Product details

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab281353-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human HK2 knockout HEK-293 cell lysate", "number":"AB281353-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK-293 cell lysate", "number":"AB281353-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Gene name
HK2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

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