Human HLA-E (HLA E) knockout A549 cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
HLA-E KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon2.
View Alternative Names
EA1.2, EA2.1, HLA 6.2, HLA class I histocompatibility antigen E alpha chain, HLA class I histocompatibility antigen E alpha chain precursor, HLA class I histocompatibility antigen alpha chain E, Lymphocyte antigen, MHC, MHC HLA E alpha 1, MHC HLA E alpha 2.1, MHC class I antigen E, Major histocompatibility complex class I E, QA1
- WB
Unknown
Western blot - Human HLA-E (HLA E) knockout A549 cell lysate (AB258453)
Lane 1 : Wild-type A549 cell lysate (20 ug)
Lane 2 : HLA-E knockout A549 cell lysate (20 ug)
Lane 3 : THP-1 cell lysate (20 ug)
Lane 4 : Jurkat cell lysate (20 ug)
ab2216 was shown to specifically react with HLA E in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267081 (knockout cell lysate ab258453) was used. Wild-type and HLA E knockout samples were subjected to SDS-PAGE. ab2216 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
false
- Sanger seq
Unknown
Sanger Sequencing - Human HLA-E (HLA E) knockout A549 cell lysate (AB258453)
Homozygous : 5 bp deletion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
![Flow Cytometry - Anti-HLA Class I antibody [W6/32] (AB22432)](https://content.abcam.com/products/images/hla-class-i-antibody-w6-32-ab22432--flow-cytometry-img428942.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com