HMGB2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 26 bp deletion in exon 2.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 26 bp deletion in exon 2.
Alternative names
C80539, HMG-2, HMGB2_HUMAN, High mobility group (nonhistone chromosomal) protein 2, High mobility group box 2, High mobility group protein 2, High mobility group protein B2
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HMGB2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 26 bp deletion in exon 2.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 26 bp deletion in exon 2.
- Concentration
Properties
- Gene name
- HMGB2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Human HMGB2 knockout HEK-293T cell lysate (ab257156)
Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: HMGB2 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - Anti-HMGB2 antibody [EPR6302] ab133540 observed at 24 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-HMGB2 antibody [EPR6302] ab133540 Anti-HMGB2 antibody [EPR6302] was shown to specifically react with HMGB2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human HMGB2 knockout HEK-293T cell line ab266358 (knockout cell lysate ab257156) was used. Wild-type and HMGB2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-HMGB2 antibody [EPR6302] ab133540 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-HMGB2 antibody [EPR6302] (Anti-HMGB2 antibody [EPR6302] ab133540) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: HMGB2 knockout HEK-293T cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Western blot - Human HMGB2 knockout HEK-293T cell lysate (ab257156)
Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: HMGB2 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - Anti-HMGB2 antibody [EPR6301] ab124670 observed at 24 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-HMGB2 antibody [EPR6301] ab124670 Anti-HMGB2 antibody [EPR6301] was shown to specifically react with HMGB2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human HMGB2 knockout HEK-293T cell line ab266358 (knockout cell lysate ab257156) was used. Wild-type and HMGB2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-HMGB2 antibody [EPR6301] ab124670 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-HMGB2 antibody [EPR6301] (Anti-HMGB2 antibody [EPR6301] ab124670) at 1/2000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: HMGB2 knockout HEK-293T cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Sanger Sequencing - Human HMGB2 knockout HEK-293T cell lysate (ab257156)
Homozygous: 26 bp deletion in exon 2
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