Human HMGN4 knockout HeLa cell lysate
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HMGN4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 31 bp deletion in exon2.
View Alternative Names
HMG17L3, HMGN4_HUMAN, High mobility group (nonhistone chromosomal) protein 17 like 3, High mobility group nucleosomal binding domain 4, High mobility group nucleosome-binding domain-containing protein 4, High mobility group protein 17 like 3, High mobility group protein N4, MGC5145, NHC, Non-histone chromosomal protein, Non-histone chromosomal protein HMG-17-like 3
- Sanger seq
Unknown
Sanger Sequencing - Human HMGN4 knockout HeLa cell lysate (AB263229)
Allele-1 : 31 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human HMGN4 knockout HeLa cell lysate (AB263229)
Allele-2 : 1 bp insertion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HMGN4 modulates transcriptional activity by altering the chromatin compaction state not working as a part of a complex. It influences how genes are turned on or off impacting cellular processes such as differentiation and proliferation. This modulation occurs through its interaction with nucleosomes positioning it as an important factor in the control of gene regulatory mechanisms across different cell types.
Pathways
Researchers study HMGN4 to understand its involvement in transcriptional regulation and DNA repair pathways. These pathways guide critical cellular activities affecting how cells respond to damage and manage genetic information. HMGN4 interacts with proteins like histone H1 an important player in facilitating chromatin remodeling which supports effective gene regulation and maintains genomic integrity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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