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AB257989

Human HNRNPA0 knockout HEK-293T cell lysate

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HNRNPA0 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon 1.

View Alternative Names

Heterogeneous nuclear ribonucleoprotein A0, HNRNPA0, hnRNP A0, HNRPA0

2 Images
Western blot - Human HNRNPA0 knockout HEK-293T cell lysate (AB257989)
  • WB

Lab

Western blot - Human HNRNPA0 knockout HEK-293T cell lysate (AB257989)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : HNRNPA0 knockout HEK293T cell lysate (20 ug)
Lane 3 : A549 cell lysate (20 ug)

ab197023 was shown to specifically react with HNRNPA0 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266314 (knockout cell lysate ab257989) was used. Wild-type and HNRNPA0 knockout samples were subjected to SDS-PAGE. ab197023 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-HNRNPA0 antibody [EP16085] (<a href='/en-us/products/primary-antibodies/hnrnpa0-antibody-ep16085-ab197023'>ab197023</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

HNRNPA0 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human HNRNPA0 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hnrnpa0-knockout-hek-293t-cell-line-ab266314'>ab266314</a>)

Lane 3:

A549 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 31 kDa

Observed band size: 32 kDa,34 kDa

false

Sanger Sequencing - Human HNRNPA0 knockout HEK-293T cell lysate (AB257989)
  • Sanger seq

Unknown

Sanger Sequencing - Human HNRNPA0 knockout HEK-293T cell lysate (AB257989)

Homozygous : 8 bp deletion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon 1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
HNRNPA0
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The heterogeneous nuclear ribonucleoprotein A0 (HNRNPA0) is a protein involved in RNA binding and regulation of RNA metabolism. Commonly referred to as HNRNPA0 it has a molecular mass close to 34 kDa. This protein is expressed in a variety of tissues including brain liver and spleen suggesting its widespread functional relevance. HNRNPA0 plays a role in the post-transcriptional regulation of gene expression by influencing mRNA stability and splicing.
Biological function summary

HNRNPA0 modulates RNA processing and is actively involved in mRNA decay and stabilization processes. It forms complexes with other hnRNP proteins and interacts with mRNA substrates to regulate their fate within the cell. This protein contributes to gene expression regulation under stress conditions by altering the stability of specific mRNAs. Such regulation is important for maintaining proper cellular responses to external stress factors.

Pathways

HNRNPA0 is involved in the p38 MAPK signaling pathway where it influences the stability of mRNAs encoding inflammatory mediators. It also plays a part in the apoptotic pathway where it interacts with proteins such as TRAF2 and apoptosis as death domain-like protein (ADRP). Through these pathways HNRNPA0 contributes to cellular stress responses inflammation and programmed cell death linking it to essential cellular processes.

HNRNPA0 has been implicated in neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) where protein aggregates may form due to dysregulation of HNRNPA0 activity. It is also associated with certain types of cancer such as leukemia where altered expression levels may impact mRNA stability and cell proliferation. Through these disease contexts HNRNPA0 interacts with proteins like TDP-43 in ALS and has connections to proto-oncogenes in cancer highlighting its potential role as a therapeutic target.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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