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AB256942

Human HNRNPA1 knockout HEK-293T cell lysate

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HNRNPA1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 4 bp insertion in exon 1.

View Alternative Names

HNRPA1, Helix-destabilizing protein, Heterogeneous nuclear ribonucleoprotein A1, Heterogeneous nuclear ribonucleoprotein A1B protein, Heterogeneous nuclear ribonucleoprotein B2 protein, Heterogeneous nuclear ribonucleoprotein core protein A1, MGC102835, Nuclear ribonucleoprotein particle A1 protein, ROA1_HUMAN, Single strand DNA binding protein UP1, Single-strand RNA-binding protein, hnRNP A1, hnRNP core protein A1

3 Images
Western blot - Human HNRNPA1 knockout HEK-293T cell lysate (AB256942)
  • WB

Lab

Western blot - Human HNRNPA1 knockout HEK-293T cell lysate (AB256942)

Lane 1 : Wild-type HEK293T cell lysate (20 μg)

Lane 2 : HNRNPA1 knockout HEK293T cell lysate (20 μg)

Lane 3 : HeLa cell lysate (20 μg)

Lanes 1-3 : Merged signal (red and green). Green - ab177152 observed at 37 kDa. Red - loading control ab8245 observed at 36 kDa.

ab177152 Anti-hnRNP A1 antibody [EPR12768] was shown to specifically react with hnRNP A1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266193 (knockout cell lysate ab256942) was used. Wild-type and hnRNP A1 knockout samples were subjected to SDS-PAGE. ab177152 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-hnRNP A1 antibody [EPR12768] (<a href='/en-us/products/primary-antibodies/hnrnp-a1-antibody-epr12768-ab177152'>ab177152</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

HNRNPA1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human HNRNPA1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hnrnpa1-knockout-hek-293t-cell-line-ab266193'>ab266193</a>)

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 23 kDa,25 kDa,33 kDa,38 kDa,53 kDa,72 kDa

Observed band size: 25 kDa,27 kDa,37 kDa,38 kDa,58 kDa,72 kDa

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Sanger Sequencing - Human HNRNPA1 knockout HEK-293T cell lysate (AB256942)
  • Sanger seq

Unknown

Sanger Sequencing - Human HNRNPA1 knockout HEK-293T cell lysate (AB256942)

Allele-2 : 4 bp insertion in exon 1

Sanger Sequencing - Human HNRNPA1 knockout HEK-293T cell lysate (AB256942)
  • Sanger seq

Unknown

Sanger Sequencing - Human HNRNPA1 knockout HEK-293T cell lysate (AB256942)

Allele-1 : 1 bp insertion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 4 bp insertion in exon 1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Sanger seq": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab256942-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human HNRNPA1 knockout HEK293T cell lysate", "number":"AB256942-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB256942-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Gene name
HNRNPA1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) also known just as hnRNP A1 is a multifunctional protein that plays important roles in RNA processing mechanisms. It is a member of the hnRNP protein family involved in the packaging of nascent pre-mRNA. hnRNP A1 has a molecular mass of approximately 34-38 kDa and shows a widespread expression in various cells and tissues. This protein is recognized for its ability to bind to RNA molecules contributing to their proper processing splicing and transport within the cellular context.
Biological function summary

HnRNP A1 is involved in RNA binding and splicing contributing to the formation of spliceosomes the complexes responsible for pre-mRNA splicing. The protein aids in alternative splicing influencing mRNA diversity and stability. hnRNP A1 interacts with other proteins in the hnRNP family remodeling ribonucleoprotein complexes and affecting their functions. These interactions ensure proper mRNA maturation impacting gene expression regulation within the cell.

Pathways

HnRNP A1 is integral to critical cellular mechanisms such as the regulation of mRNA transport and splicing. It influences the DNA damage response pathway by modulating gene expression required for cellular repair and stability. hnRNP A1 also cooperates with other RNA binding proteins like the splicing factors SR proteins to orchestrate the splicing machinery. These interactions are pivotal in maintaining cellular homeostasis and response to stress.

HnRNP A1 is notably related to neurodegenerative diseases like Amyotrophic Lateral Sclerosis (ALS) and certain types of cancer. Abnormalities in its expression or mutations can lead to disrupted cellular processes contributing to disease pathology. In ALS dysregulation of hnRNP A1 affects neuronal RNA metabolism and is associated with the TDP-43 protein a marker of neurodegeneration. In cancer hnRNP A1 influences tumor development through its role in alternative splicing and gene expression modification linking it to oncogenic signaling pathways.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Cell Lines & Lysates

AB266193

Human HNRNPA1 knockout HEK-293T cell line

Advanced Validation

Western blot - Human HNRNPA1 knockout HEK-293T cell line (AB266193)

  • 0
  • 0
Primary Antibodies

AB177152

Anti-hnRNP A1 antibody [EPR12768]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP A1 antibody [EPR12768] (AB177152)

  • 0
  • 0
Primary Antibodies

AB134916

Anti-TAF15 antibody [EPR9197(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF15 antibody [EPR9197(B)] (AB134916)

  • 5
  • 4
Proteins & Peptides

AB123212

Recombinant Human hnRNP A1 protein

SDS-PAGE - Recombinant Human hnRNP A1 protein (AB123212)

  • 0
  • 0

Product promise

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