Human HNRNPA2B1 knockout HEK-293T cell lysate
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HNRNPA2B1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 3.
View Alternative Names
HNRPA2, HNRPA2B1, HNRPB1, Heterogeneous nuclear ribonucleoprotein A2, Heterogeneous nuclear ribonucleoprotein A2/B1, Heterogeneous nuclear ribonucleoprotein B1, Heterogeneous nuclear ribonucleoproteins A2/B1, Hnrnpa2b1, Nuclear ribonucleoprotein particle A2 protein, RNP-A2, RNP-B1, ROA2_HUMAN, SNRPB1, hnRNP A2 / hnRNP B1, hnRNP A2/B1, hnRNP-A2, hnRNP-B1
- WB
Lab
Western blot - Human HNRNPA2B1 knockout HEK-293T cell lysate (AB257224)
Lane 1 : Wild-type HEK-293T (human embryonic kidney epithelial cell), whole cell lysate 20 μg
Lane 2 : hnRNP A2B1 knockout HEK-293T (human embryonic kidney epithelial cell), whole cell lysate 20 μg
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 20 μg
Lane 4 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate 20 μg
Lane 5 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate 20 μg
Blocking and diluting buffer and concentration : Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
Lanes 1- 4 : Merged signal (red and green). Green - ab259894 observed at 36/38kDa. Red - loading control ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) observed at 50 kDa.
Lanes 1-2 : ab259894 Anti-hnRNP A2B1 antibody was shown to react with hnRNP A2B1 in HEK-293T cells in Western blot. Loss of signal was observed when hnRNP A2B1 knockout cell line ab266404 (knockout cell lysate ab257224) was used. Wild-type and hnRNP A2B1 knockout samples were subjected to SDS-PAGE.
ab259894 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at 4°C overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-hnRNP A2B1 antibody [EPR24002-81] (<a href='/en-us/products/primary-antibodies/hnrnp-a2b1-antibody-epr24002-81-ab259894'>ab259894</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 2:
hnRNP A2B1 knockout HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 2:
Western blot - Human HNRNPA2B1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hnrnpa2b1-knockout-hek-293t-cell-line-ab266404'>ab266404</a>)
Lane 3:
HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 4:
A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 5:
K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 36 kDa,38 kDa
false
- WB
Lab
Western blot - Human HNRNPA2B1 knockout HEK-293T cell lysate (AB257224)
Lane 1 : Wild-type HEK293T cell lysate (20 μg)
Lane 2 : HNRNPA2B1 knockout HEK293T cell lysate (20 μg)
Lane 3 : A549 cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab31645 observed at 37 kDa. Red - loading control, ab7291 observed at 50 kDa.
ab31645 Anti-hnRNP A2B1 antibody was shown to specifically react with hnRNP A2B1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266404 (knockout cell lysate ab257224) was used. Wild-type and hnRNP A2B1 knockout samples were subjected to SDS-PAGE. ab31645 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti- Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-hnRNP A2B1 antibody (<a href='/en-us/products/primary-antibodies/hnrnp-a2b1-antibody-ab31645'>ab31645</a>) at 1/500 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
HNRNPA2B1 knockout HEK293T cell lysate at 20 µg
Lane 3:
A549 cell lysate at 20 µg
Predicted band size: 37 kDa
Observed band size: 37 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human HNRNPA2B1 knockout HEK-293T cell lysate (AB257224)
Homozygous : 2 bp deletion in exon 3
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HnRNP A2B1 contributes to alternative splicing RNA stability and translational regulation. This protein typically associates with complex assemblies that manage RNA processing and transport mechanisms. It interacts with other proteins within the heterogeneous nuclear ribonucleoprotein family collaborating in the regulation of mRNA cycling between the nucleus and cytoplasm affecting gene expression patterns.
Pathways
HnRNP A2B1 exhibits critical involvement in the mRNA splicing pathway and RNA transport pathways. It frequently interacts with proteins such as hnRNP A1 and hnRNP C where they collectively influence pre-mRNA processing and splicing dictating proper RNA maturation and cell function. These pathways are important for maintaining cellular homeostasis and responding to cellular signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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