Human HNRNPA3 knockout HEK-293T cell lysate
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HNRNPA3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 0 bp deletion in exon1 and 32 bp deletion in exon1.
View Alternative Names
FBRNP, HNRPA3, Heterogeneous nuclear ribonucleoprotein A3
- Sanger seq
Unknown
Sanger Sequencing - Human HNRNPA3 knockout HEK-293T cell lysate (AB257991)
Allele-1 : 32 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human HNRNPA3 knockout HEK-293T cell lysate (AB257991)
Allele-2 : 0 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HNRPA3 is key in regulating RNA splicing by forming complexes with components such as hnRNPs a diverse group of RNA-binding proteins. These complexes contribute to the modification and alternative splicing of pre-mRNA which is essential for generating protein diversity. HNRPA3 stabilizes mRNA and influences the transcriptional activity impacting gene expression regulation. Its interactions ensure mRNA molecules are correctly processed and available for translation.
Pathways
HNRPA3 actively participates in the spliceosome pathway which is responsible for the splicing of pre-mRNA. Additionally it plays a role in the mRNA surveillance pathway ensuring the fidelity of RNA molecules. Through these pathways HNRPA3 collaborates with proteins such as HNRNPA1 and SRSF1 which also facilitate RNA binding and processing activities. This coordination is critical for maintaining cellular homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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