Human HNRNPAB knockout HEK-293T cell lysate
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HNRNPAB KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2.
View Alternative Names
ABBP-1, APOBEC1-binding protein 1, Apolipoprotein B mRNA editing enzyme catalytic polypeptide 1- protein 1, FLJ40338, HNRNPAB, Heterogeneous nuclear ribonucleoprotein A/B, ROAA_HUMAN, hnRNP A/B, hnRNP type A/B protein
- WB
Lab
Western blot - Human HNRNPAB knockout HEK-293T cell lysate (AB257467)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : HNRNPAB knockout HEK293T cell lysate (20 ug)
Lane 3 : K-562 cell lysate (20 ug)
Lane 4 : Jurkat cell lysate (20 ug)
ab199724 was shown to specifically react with HNRPAB in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266459 (knockout cell lysate ab257467) was used. Wild-type and HNRPAB knockout samples were subjected to SDS-PAGE. ab199724 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-HNRPAB antibody [EPR16944] (<a href='/en-us/products/primary-antibodies/hnrpab-antibody-epr16944-ab199724'>ab199724</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
HNRNPAB knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human HNRNPAB knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hnrnpab-knockout-hek-293t-cell-line-ab266459'>ab266459</a>)
Lane 3:
K-562 cell lysate at 20 µg
Lane 4:
Jurkat cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 48 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human HNRNPAB knockout HEK-293T cell lysate (AB257467)
Homozygous : 5 bp deletion in exon 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HNRPAB influences the splicing and transport of pre-mRNA impacting the maturation process of mRNA. It collaborates with other hnRNPs and splicing factors to modulate gene expression. The protein sometimes forms part of larger complexes that are essential for RNA processing. This complex helps to stabilize RNA molecules assisting in their proper transport and translation in the cytoplasm.
Pathways
HNRPAB is involved in the regulation of RNA metabolism and processing pathways. It forms a network with other proteins like hnRNP F and hnRNP H impacting gene expression by influencing mRNA stability and availability. In conjunction with these proteins HNRPAB plays a role in the signal transduction pathways affecting cellular responses and adaptations.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-HNRPAB antibody [EPR16944] (AB199724)](https://content.abcam.com/products/images/hnrpab-antibody-epr16944-ab199724--western-blot-img61879.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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