Human HNRNPDL knockout HeLa cell lysate
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HNRNPDL KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 38 bp deletion in exon 1 and 47 bp deletion in exon 1.
View Alternative Names
A+U-rich element RNA binding factor, AA407431, AA959857, AU-rich element RNA-binding factor, D5Ertd650e, D5Wsu145e, HNRDL_HUMAN, HNRNP, Heterogeneous nuclear ribonucleoprotein D like protein, Heterogeneous nuclear ribonucleoprotein D-like, JKT41-binding protein, JKTBP, JKTBP2, MGC125262, Protein laAUF1, hnHNRP DL, hnRNP D-like, hnRNP DL, hnRPD like protein, laAUF1
- Sanger seq
Unknown
Sanger Sequencing - Human HNRNPDL knockout HeLa cell lysate (AB258905)
Allele-1 : 47 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human HNRNPDL knockout HeLa cell lysate (AB258905)
Allele-2 : 38 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HnRNP D-like protein participates in regulating gene expression post-transcriptionally. It often forms a complex with other hnRNP proteins enabling the modulation of RNA metabolic processes. This protein binds to RNA through its nucleic acid-binding domains influencing the mRNA stability and pre-mRNA processing. It affects various cellular processes by regulating gene transcripts vital for maintaining normal cellular functions.
Pathways
HnRNP D-like protein is part of key processes that influence mRNA splicing and transport. It is involved in the mRNA surveillance pathway that monitors and degrades abnormal mRNA molecules. HNRPDL also interacts with other proteins like HNRNPA1 in these pathways showing its importance in RNA regulation and cellular homeostasis. Its function in these pathways suggests it plays a role in ensuring the accuracy of mRNA synthesis and processing.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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