Human HNRNPR knockout HEK-293T cell lysate
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HNRNPR KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 2 bp deletion in exon 3.
View Alternative Names
FLJ25714, HNRPR_HUMAN, Heterogeneous nuclear ribonucleoprotein R, hnRNP-R
- WB
Lab
Western blot - Human HNRNPR knockout HEK-293T cell lysate (AB257992)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : HNRNPR knockout HEK-293T cell lysate 20 μg
Lane 3 : MOLT-4 cell lysate 20 μg
Lane 4 : Caco-2 cell lysate 20 μg
False colour image of Western blot : Anti-hnRNP R antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab151251 was shown to bind specifically to hnRNP R. A band was observed at 78 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in HNRNPR knockout cell line ab266723 (knockout cell lysate ab257992). To generate this image, wild-type and HNRNPR knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-hnRNP R antibody (<a href='/en-us/products/primary-antibodies/hnrnp-r-antibody-ab151251'>ab151251</a>) at 1/500 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
HNRNPR knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human HNRNPR knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hnrnpr-knockout-hek-293t-cell-line-ab266723'>ab266723</a>)
Lane 3:
MOLT-4 cell lysate at 20 µg
Lane 4:
Caco-2 cell lysate at 20 µg
Predicted band size: 71 kDa
Observed band size: 78 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human HNRNPR knockout HEK-293T cell lysate (AB257992)
Allele-1 : 2 bp deletion in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human HNRNPR knockout HEK-293T cell lysate (AB257992)
Allele-2 : 1 bp deletion in exon 3
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HnRNP R contributes to several cellular tasks related to gene expression regulation. This protein interacts with other hnRNPs to form core components of ribonucleoprotein complexes. It participates in modulating RNA transport from the nucleus to the cytoplasm and facilitates mRNA translation. Additionally hnRNP R associates with other proteins and RNA molecules strengthening the cellular response to environmental cues.
Pathways
HnRNP R acts decisively in several gene expression and RNA processing pathways. It is involved in the mRNA splicing pathway where it interacts with splicing machinery to process precursor mRNAs into mature mRNAs. hnRNP R also influences the Wnt signaling pathway by regulating the stability and localization of mRNAs involved in this pathway. Moreover it interacts with proteins like hnRNP A1 which further aids its functional capabilities in these pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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