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AB257993

Human HNRNPUL1 (E1B-AP5) knockout HEK-293T cell lysate

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HNRNPUL1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 3 and Insertion of the selection cassette in exon 3.
3 Images
Western blot - Human HNRNPUL1 (E1B-AP5) knockout HEK-293T cell lysate (AB257993)
  • WB

Unknown

Western blot - Human HNRNPUL1 (E1B-AP5) knockout HEK-293T cell lysate (AB257993)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : HNRNPUL1 knockout HEK293T cell lysate (20 ug)
Lane 3 : Raji cell lysate (20 ug)

ab68480 was shown to specifically react with E1B-AP5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266142 (knockout cell lysate ab257993) was used. Wild-type and E1B-AP5 knockout samples were subjected to SDS-PAGE. ab68480 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-E1B-AP5 antibody [EP2633Y] (<a href='/en-us/products/primary-antibodies/e1b-ap5-antibody-ep2633y-ab68480'>ab68480</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

HNRNPUL1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human HNRNPUL1 (E1B-AP5) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hnrnpul1-e1b-ap5-knockout-hek-293t-cell-line-ab266142'>ab266142</a>)

Lane 3:

Raji cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 96 kDa

Observed band size: 96 kDa

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Sanger Sequencing - Human HNRNPUL1 (E1B-AP5) knockout HEK-293T cell lysate (AB257993)
  • Sanger seq

Unknown

Sanger Sequencing - Human HNRNPUL1 (E1B-AP5) knockout HEK-293T cell lysate (AB257993)

Allele-1 : Insertion of the selection cassette in exon 3

Sanger Sequencing - Human HNRNPUL1 (E1B-AP5) knockout HEK-293T cell lysate (AB257993)
  • Sanger seq

Unknown

Sanger Sequencing - Human HNRNPUL1 (E1B-AP5) knockout HEK-293T cell lysate (AB257993)

Allele-2 : 16 bp deletion in exon 3

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 3 and Insertion of the selection cassette in exon 3.

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Primary Antibodies

AB68480

Anti-E1B-AP5 antibody [EP2633Y]

RabMAb|KO Validated

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Cell Lines & Lysates

AB266142

Human HNRNPUL1 (E1B-AP5) knockout HEK-293T cell line

Advanced Validation

Western blot - Human HNRNPUL1 (E1B-AP5) knockout HEK-293T cell line (AB266142)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
HNRNPUL1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The E1B-AP5 protein also known as hnRNPUL1 serves mechanical functions by binding to RNA and DNA and it acts as a regulator in transcription and mRNA processing. This protein weighs approximately 88 kDa. Researchers have identified its presence in the nucleus of different cell types reflecting its central role in nucleic acid metabolism. The protein can also intervene in alternative splicing events and influence mRNA stability making it a significant player within the cell's genetic expression regulation matrix.
Biological function summary

E1B-AP5 integrates into cellular processes as a part of the heterogeneous nuclear ribonucleoproteins complex which facilitates various RNA processing mechanisms. It plays an active role in chromatin structure modulation and RNA transport fostering proper gene expression and maintaining cellular homeostasis. Besides supporting the transcription machinery E1B-AP5 engages in RNA export and surveillance where its presence determines the fate of newly synthesized RNAs.

Pathways

Studies have shown that E1B-AP5 is involved in pathways such as the mRNA surveillance and splicing pathways. It collaborates with proteins like HNRNPA1 and HNRNPC in orchestrating the correct assembly and exportation of RNA transcripts. By participating in these critical pathways E1B-AP5 ensures the fidelity of gene expression and supports the cellular stress response mechanisms contributing to proper cellular function and adaptability.

Abnormalities in E1B-AP5 expression correlate with oncogenic transformations and neurodegenerative conditions. In cancer for example dysregulation of E1B-AP5 affects RNA processing and can lead to tumor development with its interaction noted alongside the protein p53 in the context of cellular proliferation control. Understanding E1B-AP5’s role in these diseases might provide insights into therapeutic targets particularly where RNA processing and tumor suppressor pathways are implicated.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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