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AB257996

Human HR (Hairless) knockout HeLa cell lysate

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HR KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 3.

View Alternative Names

ALUNC, HAIR_HUMAN, HSA277165, Hairless protein, Host range, Protein hairless

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Sanger Sequencing - Human HR (Hairless) knockout HeLa cell lysate (AB257996)
  • Sanger seq

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Sanger Sequencing - Human HR (Hairless) knockout HeLa cell lysate (AB257996)

Homozygous : Insertion of the selection cassette in exon 3

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 3.

Disease

Adenocarcinoma

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
HR
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hairless also known as HR is a nuclear receptor corepressor that plays an important role in hair follicle development. With a molecular mass of approximately 130 kDa it acts mechanistically by interacting with transcription factors to regulate gene expression. Hairless is expressed mainly in the skin particularly in the hair follicles but it can also be found in other tissues like the brain adipose tissue and testes. Its ability to localize in the nucleus allows Hairless to directly control chromatin structure and transcriptional activity.
Biological function summary

Hairless is involved in the regulation of hair cycling and differentiation. It functions as part of a larger repressor complex recruiting histone deacetylases to histones which results in gene silencing. This activity is important for the transition between different phases of the hair growth cycle such as anagen and telogen phases. Hairless also plays a role in repressing certain genes involved in epidermal differentiation thereby maintaining stem cell populations necessary for continuous hair growth.

Pathways

Hairless significantly impacts the Wnt signaling pathway and the Notch signaling pathway. In the Wnt pathway Hairless collaborates with proteins like beta-catenin to influence follicle morphogenesis and hair cycle progression. In the Notch signaling cascade Hairless interacts with Notch receptors regulating cell fate decisions during hair development. These pathways are essential for proper hair follicle regeneration and maintenance highlighting Hairless's critical role in hair biology.

Hairless has been implicated in alopecia universalis a condition characterized by complete hair loss. Mutations in the Hairless gene can result in faulty protein function causing disruption in hair development and cycling. Furthermore Hairless has ties to Marie Unna hereditary hypotrichosis a genetic disorder leading to sparse hair and scalp abnormalities. The implicated pathways also involve proteins such as Notch1 and beta-catenin which are associated with defects in hair follicle development and structure.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

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