Human HSPB1 (Hsp27) knockout HeLa cell lysate
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HSPB1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
View Alternative Names
28 kDa heat shock protein, CMT2F, DKFZp586P1322, Estrogen-regulated 24 kDa protein, HEL-S-102, HMN2B, HS.76067, HSP 27, HSPB1_HUMAN, Heat Shock Protein 27, Heat shock 25kDa protein 1, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, Hsp 25, Hsp 28, SRP27, Stress-responsive protein 27, epididymis secretory protein Li 102, heat shock protein family B (small) member 1
- WB
Lab
Western blot - Human HSPB1 (Hsp27) knockout HeLa cell lysate (AB256945)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : Hsp27 knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab109376 observed at 23 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109376 Anti-Hsp27 antibody [EPR5477] was shown to specifically react with Hsp27 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261738 (knockout cell lysate ab256945) was used. Wild-type and Hsp27 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109376 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Hsp27 antibody [EPR5477] (<a href='/en-us/products/primary-antibodies/hsp27-antibody-epr5477-ab109376'>ab109376</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
HSPB1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human HSPB1 (Hsp27) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-hspb1-hsp27-knockout-hela-cell-line-ab261738'>ab261738</a>)
Predicted band size: 23 kDa
Observed band size: 23 kDa
false
- WB
Lab
Western blot - Human HSPB1 (Hsp27) knockout HeLa cell lysate (AB256945)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : Hsp27 knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab62339 observed at 23 kDa. Red - loading control ab8245 observed at 37 kDa.
ab62339 Anti-Hsp27 antibody [EP1724Y] was shown to specifically react with Hsp27 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261738 (knockout cell lysate ab256945) was used. Wild-type and Hsp27 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab62339 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Hsp27 antibody [EP1724Y] (<a href='/en-us/products/primary-antibodies/hsp27-antibody-ep1724y-ab62339'>ab62339</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
HSPB1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human HSPB1 (Hsp27) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-hspb1-hsp27-knockout-hela-cell-line-ab261738'>ab261738</a>)
Predicted band size: 23 kDa
Observed band size: 23 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human HSPB1 (Hsp27) knockout HeLa cell lysate (AB256945)
Homozygous : 1 bp insertion in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.
Pathways
Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
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