Human HTR3D (5HT3D Receptor) knockout A549 cell lysate
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HTR3D KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6 and 5 bp deletion in exon6.
View Alternative Names
5 HT3 D, 5 hydroxytryptamine receptor 3D, 5-hydroxytryptamine receptor 3 subunit D, HTR3D, MGC119636, MGC119637, Serotonin 5-HT-3D receptor, Serotonin receptor 3D
- Sanger seq
Unknown
Sanger Sequencing - Human HTR3D (5HT3D Receptor) knockout A549 cell lysate (AB263232)
Allele-2 : 1 bp insertion in exon6
- Sanger seq
Unknown
Sanger Sequencing - Human HTR3D (5HT3D Receptor) knockout A549 cell lysate (AB263232)
Allele-1 : 5 bp deletion in exon6
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The 5HT3D Receptor functions as a component of the ligand-gated ion channel complex allowing the passage of cations including sodium (Na+) and calcium (Ca2+) upon serotonin binding. This action affects neuronal excitability and neurotransmitter release. The receptor plays a significant role in intestinal motility modulating gut motility and in the central nervous system where it contributes to emotions and cognitive processes.
Pathways
The 5HT3D Receptor is involved in the serotonergic synapse pathway which is important for transmitting signals in the nervous system. The receptor works alongside other serotonin receptors such as 5HT3A influencing neurotransmitter release and neuronal excitability. It also interacts within the GABAergic synapse pathway where it may affect inhibitory neurotransmission alongside the GABA receptors. These interactions can impact mood regulation and cognitive functions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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