Human IDH3G knockout HEK-293T cell lysate
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IDH3G KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon2.
View Alternative Names
H IDHG, IDH gamma, IDH3G_HUMAN, Isocitrate dehydrogenase 3 (NAD+) gamma, Isocitrate dehydrogenase [NAD] subunit gamma, Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, Isocitric dehydrogenase, Isocitric dehydrogenase subunit gamma, NAD (H) specific isocitrate dehydrogenase gamma subunit, NAD(+) specific ICDH, NAD(+)-specific ICDH subunit gamma, NAD+ specific ICDH, OTTHUMP00000025984, OTTHUMP00000025985, OTTHUMP00000025987, OTTHUMP00000025988, OTTHUMP00000214764, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human IDH3G knockout HEK-293T cell lysate (AB263233)
Homozygous : Insertion of the selection cassette in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IDH3G is part of the IDH3 enzyme complex which contains multiple subunits and functions within mitochondria. This complex plays a critical role in the tricarboxylic acid (TCA) cycle also known as the Krebs cycle. This cycle is fundamental for energy production as it supplies reducing equivalents in the form of NADH which drive ATP synthesis through oxidative phosphorylation. The proper functioning of IDH3G contributes to efficient cellular respiration and energy homeostasis.
Pathways
The function of IDH3G connects to the TCA cycle and the energy metabolism pathway. Within the TCA cycle IDH3G's enzymatic activity ensures the continuation of the cycle and maintains the production of NADH. This is key for the generation of ATP in oxidative phosphorylation. The IDH3 complex works closely with the other components of the TCA cycle including succinate dehydrogenase and citrate synthase ensuring the continuity and regulation of metabolic flux in energy production pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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