Human IDS (Iduronate 2 sulfatase/SIDS) knockout HeLa cell lysate
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IDS KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon4 and 1 bp insertion in exon4.
View Alternative Names
AW214631, Alpha-L-iduronate sulfate sulfatase, IDS_HUMAN, Iduronate 2 sulfatase, Iduronate 2 sulfatase 42 kDa chain, Iduronate 2-sulfatase 14 kDa chain, Iduronate sulfatase, Idursulfase, MPS2, RP23-29M4.1, SIDS
- Sanger seq
Unknown
Sanger Sequencing - Human IDS (Iduronate 2 sulfatase/SIDS) knockout HeLa cell lysate (AB258462)
Allele-2 : 1 bp insertion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human IDS (Iduronate 2 sulfatase/SIDS) knockout HeLa cell lysate (AB258462)
Allele-1 : 10 bp deletion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IDS functions to ensure proper breakdown and recycling of GAGs preventing their excessive accumulation. Although IDS acts independently its proper functioning complements other enzymes within the lysosomal degradation system. This lysosomal catabolic activity helps maintain cellular homeostasis as GAGs need controlled degradation for effective cell and tissue functions.
Pathways
IDS plays an important role in the lysosomal degradation pathway. By participating in the catabolism of GAGs IDS works alongside other enzymes such as alpha-L-iduronidase. This interaction is part of the larger lysosomal storage and cellular turnover pathways. These pathways are essential for cellular regulation and help prevent the buildup of undegraded molecules that can disrupt cellular functions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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