Human IER2 knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
IER2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2.
View Alternative Names
ChxI protein homolog, ETR101, IER2_HUMAN, Immediate early protein, Immediate early response 2, Immediate early response gene 2 protein, MGC111472, MGC15265, Pip92, Protein ETR101, T lymphocyte activated protein
- Sanger seq
Unknown
Sanger Sequencing - Human IER2 knockout HeLa cell lysate (AB263234)
Allele-2 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human IER2 knockout HeLa cell lysate (AB263234)
Allele-1 : 1 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IER2 influences cell proliferation and differentiation. It interacts with several signaling pathways indicating its role in controlling cell cycle progression. Scientists have discussed IER2's involvement in cellular stress responses acting independently rather than as part of a larger protein complex. These activities help the protein regulate gene expression temporarily an important element in maintaining cellular homeostasis.
Pathways
IER2 functions within signaling networks essential for mitogenic responses and stress-activated MAPK pathways. It shares pathways with proteins such as c-Fos and c-Jun which are part of the AP-1 transcription factor complex. These connections position IER2 as a participant in pathways that manage cellular responses to external stimuli and internal signals important for cell growth and survival.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com