Human IFI27 knockout A549 cell lysate
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IFI27 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 146 bp deletion in exon4.
View Alternative Names
2310061N23Rik, FAM 14D, IFI27_HUMAN, ISG 12, ISG12(a), ISG12(a) protein, ISG12A, Interferon alpha-induced 11.5 kDa protein, Interferon alpha-inducible protein 27, Interferon inducible protein 27, Interferon-stimulated gene 12a protein, interferon alpha-inducible protein 27, mitochondrial, interferon-stimulated gene 12, mitochondrial, p27
- Sanger seq
Unknown
Sanger Sequencing - Human IFI27 knockout A549 cell lysate (AB258463)
Homozygous : 146 bp deletion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IFI27 plays a significant role in mediating apoptosis the programmed cell death essential for normal cellular turnover and homeostasis. Although it does not typically form part of a larger protein complex its induction by interferons implicates it in antiviral defense mechanisms. Its expression can lead to changes in mitochondrial membrane potential ultimately affecting cell survival.
Pathways
IFI27 participates in important pathways related to immunity and apoptosis. Particularly it engages in the interferon signaling pathway influencing the cellular defense against viral infections. It interacts with other proteins like STAT1 and STAT2 which are key transcription factors in the interferon signaling cascade modulating downstream antiviral responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
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Product promise
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