IFI44 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 7 bp deletion in exon2.
Images
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 7 bp deletion in exon2.
Alternative names
IFI44_HUMAN, Interferon induced hepatitis C associated microtubular aggregate protein, Interferon induced hepatitis C associated microtubular aggregate protein (44kD), Interferon-induced protein 44, MTAP 44, Microtubule-associated protein 44, OTTHUMP00000011293, TBC/LysM-associated domain containing 5, TLDC5, hepatitis C-associated microtubular aggregate protein (44kD), p44
What's included?
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IFI44 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 7 bp deletion in exon2.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 7 bp deletion in exon2.
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- IFI44
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IFI44 also known as Interferon-Induced Protein 44 is a protein encoded by the IFI44 gene. This protein has a molecular mass of approximately 49 kDa. IFI44 expresses in various tissues with elevated levels observed in immune-related cells such as leukocytes. Mechanically IFI44 is known to play a role in the antiviral defense mechanisms of cells often upregulated in response to interferon stimuli. It interacts with cytoskeleton elements to mediate its cellular functions.
- Biological function summary
IFI44 participates in cellular defense against viral infections by enhancing the immune response. This protein does not function independently but forms part of interferon-stimulated gene complex working together with other proteins to combat virus replication. By regulating this complex IFI44 influences cell signaling and modulates antiviral pathways contributing to the suppression of viral propagation within host cells.
- Pathways
IFI44 functions prominently within the interferon signaling pathway and the innate immune response pathway. It collaborates closely with other interferon-stimulated proteins such as ISG15 and MX1 which are engaged in the virus suppression. This cooperation helps orchestrate a swift immune response to external pathogenic threats by inhibiting viral gene expression and replication processes.
- Associated diseases and disorders
IFI44 plays an important role in conditions associated with viral infections such as Hepatitis C and influenza. The protein’s interaction with viral defense proteins like ISG15 is significant in these disorders. Its expression levels often correlate with the severity of the disease serving as a biomarker for the infection state. Understanding IFI44’s precise role in these diseases could lead to novel therapeutic approaches and better management of viral infections.
Product promise
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2 product images
Sanger Sequencing - Human IFI44 knockout A549 cell lysate (ab258913)
Allele-1: 14 bp deletion in exon2
Sanger Sequencing - Human IFI44 knockout A549 cell lysate (ab258913)
Allele-2: 7 bp deletion in exon2
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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