Human IFI44 knockout A549 cell lysate
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IFI44 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 7 bp deletion in exon2.
View Alternative Names
IFI44_HUMAN, Interferon induced hepatitis C associated microtubular aggregate protein, Interferon induced hepatitis C associated microtubular aggregate protein (44kD), Interferon-induced protein 44, MTAP 44, Microtubule-associated protein 44, OTTHUMP00000011293, TBC/LysM-associated domain containing 5, TLDC5, hepatitis C-associated microtubular aggregate protein (44kD), p44
- Sanger seq
Unknown
Sanger Sequencing - Human IFI44 knockout A549 cell lysate (AB258913)
Allele-1 : 14 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human IFI44 knockout A549 cell lysate (AB258913)
Allele-2 : 7 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IFI44 participates in cellular defense against viral infections by enhancing the immune response. This protein does not function independently but forms part of interferon-stimulated gene complex working together with other proteins to combat virus replication. By regulating this complex IFI44 influences cell signaling and modulates antiviral pathways contributing to the suppression of viral propagation within host cells.
Pathways
IFI44 functions prominently within the interferon signaling pathway and the innate immune response pathway. It collaborates closely with other interferon-stimulated proteins such as ISG15 and MX1 which are engaged in the virus suppression. This cooperation helps orchestrate a swift immune response to external pathogenic threats by inhibiting viral gene expression and replication processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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