Human IFIT2 knockout A549 cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human IFIT2 knockout A549 cell lysate (AB258919)
Allele-2 : 4 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human IFIT2 knockout A549 cell lysate (AB258919)
Allele-1 : 5 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human IFIT2 knockout A549 cell lysate (AB258919)
Allele-3 : 2 bp deletion in exon2
Complementary Products
Primary Antibodies
AB305231
Anti-IFIT2 antibody [EPR26404-119]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT2 antibody [EPR26404-119] (AB305231)](https://content.abcam.com/products/images/ifit2-antibody-epr26404-119-ab305231--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403829.jpg)
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Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein exerts its influence by binding to RNA molecules lacking 2'-O-methylation a modification that is usually absent in viral RNA. IFIT2 works as both a solo entity and as part of a larger complex that includes other members like IFIT1 and IFIT3. The coordination among these proteins helps modulate the immune response influencing protein synthesis regulation in cells that are under viral attack.
Pathways
IFIT2 plays an essential role in the innate immune response particularly the interferon signaling pathway. It contributes to this pathway through its interactions with other proteins ensuring the rapid activation of antiviral states. Furthermore IFIT2 also connects to the toll-like receptor (TLR) signaling pathway playing a part in recognizing viral components. Through these pathways IFIT2 typically associates with proteins like TLR3 which facilitate detection and response to viral pathogens.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com