IFIT5 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon2 and 11 bp deletion in exon2 and 13 bp deletion in exon2.
Images
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon2 and 11 bp deletion in exon2 and 13 bp deletion in exon2.
Alternative names
FLJ53857, FLJ92678, IFIT5_HUMAN, Interferon-induced protein with tetratricopeptide repeats 5, RI58, Retinoic acid and interferon inducible protein (58kD), Retinoic acid- and interferon-inducible 58 kDa protein
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IFIT5 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon2 and 11 bp deletion in exon2 and 13 bp deletion in exon2.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon2 and 11 bp deletion in exon2 and 13 bp deletion in exon2.
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- IFIT5
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IFIT5 also known as Interferon-induced protein with tetratricopeptide repeats 5 is a protein with a molecular mass of approximately 56 kDa. This protein is expressed mainly in the cytoplasm where it functions by specifically binding to single-stranded RNAs lacking 2'-O-methylation at their 5'-cap structure. This recognition allows IFIT5 to distinguish self from non-self RNA an important element in immune defense mechanisms. Its ability to sense and bind to viral RNA makes it an important player in the host's innate immune response.
- Biological function summary
IFIT5 is involved in antiviral defense and immune response regulation. It does not function as part of a multi-protein complex but interacts with various signaling molecules to amplify immune responses. By selectively binding to foreign RNA IFIT5 can inhibit viral replication and modulate type I interferon signaling pathways. It plays a significant role in restricting viral protein synthesis giving the host organism time to mount an adaptive immune response.
- Pathways
IFIT5 is closely associated with the interferon signaling pathway and the innate immune response pathway. Through its interactions it affects the Jak-STAT signaling pathway which is important for the induction of antiviral states in cells. IFIT5 also has functional links with other proteins such as RIG-I an important sensor in the detection of viral RNAs. Together they provide a robust early immune defense by facilitating cytokine production and ensuring rapid response to infections.
- Associated diseases and disorders
IFIT5 has been linked to viral infections and certain inflammatory conditions. Its role in antiviral defense connects it to diseases such as hepatitis C where the protein's influence on interferon action can affect viral persistence and patient response to treatment. Additionally IFIT5 interaction with proteins like PKR moves it to the context of autoimmune disorders where overactive or dysregulated immune responses can lead to chronic inflammation and tissue damage.
Product promise
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Terms & Conditions.
3 product images
Sanger Sequencing - Human IFIT5 knockout A549 cell lysate (ab258920)
Allele-3: 10 bp deletion in exon2
Sanger Sequencing - Human IFIT5 knockout A549 cell lysate (ab258920)
Allele-1: 13 bp deletion in exon2
Sanger Sequencing - Human IFIT5 knockout A549 cell lysate (ab258920)
Allele-2: 11 bp deletion in exon2
Downloads
Product protocols
- Visit the general protocols
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