Human IGF1R (IGF1 Receptor) knockout HeLa cell lysate
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IGF1R KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 2 and 1 bp deletion in exon 2.
View Alternative Names
CD221, CD221 antigen, IGF 1 receptor, IGF-I receptor, IGF1R_HUMAN, IGFIR, IGFIRC, IGFR, Insulin like growth factor 1 receptor, Insulin like growth factor 1 receptor precursor, Insulin-like growth factor 1 receptor beta chain, Insulin-like growth factor I receptor, JTK13, MGC142170, MGC142172, MGC18216, Soluble IGF1R variant 1, Soluble IGF1R variant 2
- WB
Lab
Western blot - Human IGF1R (IGF1 Receptor) knockout HeLa cell lysate (AB256951)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : IGF1R knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab263907 observed at 100 kDa. Red - loading control ab8245 observed at 37 kDa.
ab263907 Anti-IGF1 Receptor antibody [EPR23027-80] was shown to specifically react with IGF1 Receptor in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264801 (knockout cell lysate ab256951) was used. Wild-type and IGF1 Receptor knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab263907 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-IGF1 Receptor antibody [EPR23027-80] (<a href='/en-us/products/primary-antibodies/igf1-receptor-antibody-epr23027-80-ab263907'>ab263907</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
IGF1R knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human IGF1R (IGF1 Receptor) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-igf1r-igf1-receptor-knockout-hela-cell-line-ab264801'>ab264801</a>)
Predicted band size: 154 kDa
Observed band size: 100 kDa
false
- WB
Lab
Western blot - Human IGF1R (IGF1 Receptor) knockout HeLa cell lysate (AB256951)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : IGF1R knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab182408 observed at 100 kDa. Red - loading control ab8245 observed at 37 kDa.
ab182408 Anti-IGF1 Receptor antibody [EPR19322] was shown to specifically react with IGF1 Receptor in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264801 (knockout cell lysate ab256951) was used. Wild-type and IGF1 Receptor knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab182408 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-IGF1 Receptor antibody [EPR19322] (<a href='/en-us/products/primary-antibodies/igf1-receptor-antibody-epr19322-ab182408'>ab182408</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
IGF1R knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human IGF1R (IGF1 Receptor) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-igf1r-igf1-receptor-knockout-hela-cell-line-ab264801'>ab264801</a>)
Predicted band size: 154 kDa
Observed band size: 100 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human IGF1R (IGF1 Receptor) knockout HeLa cell lysate (AB256951)
Allele-1 : 13 bp deletion in exon 2; Allele-2 : 1 bp deletion in exon 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IGF1R contributes to several cellular processes including cell proliferation differentiation and survival. This receptor forms a complex upon ligand binding and undergoes autophosphorylation to activate intracellular signaling cascades. IGF1R activation recruits and phosphorylates insulin receptor substrates enabling the downstream signaling pathways that promote cell growth and survival. Its function in cellular responses makes it a focal point in understanding cell biology.
Pathways
IGF1R holds a central position in the PI3K/AKT and MAPK signaling cascades. These pathways play significant roles in cellular growth proliferation and survival. IGF1R phosphorylates various downstream effectors such as IRS-1 and Shc linking it to the activation of AKT and ERK respectively. Therefore it shares pathways with related proteins like the insulin receptor highlighting its importance in mediating similar biological responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB182408
Anti-IGF1 Receptor antibody [EPR19322]
RabMAb|Recombinant|KO Validated|20ul selling size
![Immunocytochemistry/ Immunofluorescence - Anti-IGF1 Receptor antibody [EPR19322] (AB182408)](https://content.abcam.com/products/images/igf1-receptor-antibody-epr19322-ab182408--immunocytochemistry-immunofluorescence-img58812.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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