IKBKG KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 2 and 1 bp insertion in exon 2.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 2 and 1 bp insertion in exon 2.
Alternative names
AMCBX1, FIP-3, Fip3p, I kappa B kinase gamma, I-kappa-B kinase subunit gamma, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, IkB kinase gamma subunit, IkB kinase subunit gamma, IkB kinase-associated protein 1, Ikbkg, Incontinentia pigmenti, Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase gamma, Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, gamma, Inhibitor of nuclear factor kappa-B kinase subunit gamma, NEMO_HUMAN, NF-kappa-B essential modifier, NF-kappa-B essential modulator, ZC2HC9
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IKBKG KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 2 and 1 bp insertion in exon 2.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 2 and 1 bp insertion in exon 2.
- Concentration
Properties
- Gene name
- IKBKG
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
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4 product images
Western blot - Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell lysate (ab257153)
Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: IKBKG knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - Anti-IKK gamma/NEMO antibody [EPR14660] ab188569 observed at 48 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-IKK gamma/NEMO antibody [EPR14660] ab188569 Anti-IKK gamma/NEMO antibody [EPR14660] was shown to specifically react with IKK gamma/NEMO in wild-type HEK-293T cells in western blot. The band observed in the knockout cell line Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell line ab266674 (knockout cell lysate ab257153) lane below 48kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and IKK gamma/NEMO knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-IKK gamma/NEMO antibody [EPR14660] ab188569 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (Anti-IKK gamma/NEMO antibody [EPR14660] ab188569) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: IKBKG knockout HEK-293T cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Western blot - Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell lysate (ab257153)
Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: IKBKG knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - Anti-IKK gamma/NEMO antibody [EPR16629] ab178872 observed at 48 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-IKK gamma/NEMO antibody [EPR16629] ab178872 Anti-IKK gamma/NEMO antibody [EPR16629] was shown to specifically react with IKK gamma/NEMO in wild-type HEK-293T cells in western blot. The band observed in the knockout cell line Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell line ab266674 (knockout cell lysate ab257153) lane below 48kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and IKK gamma/NEMO knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-IKK gamma/NEMO antibody [EPR16629] ab178872 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (Anti-IKK gamma/NEMO antibody [EPR16629] ab178872) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: IKBKG knockout HEK-293T cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Sanger Sequencing - Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell lysate (ab257153)
Allele-2: 1 bp insertion in exon 2
Sanger Sequencing - Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell lysate (ab257153)
Allele-1: 13 bp deletion in exon 2
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