Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell lysate
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- WB
Lab
Western blot - Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell lysate (AB257153)
Lane 1 : Wild-type HEK-293T cell lysate (20µg)
Lane 2 : IKBKG knockout HEK-293T cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab188569 observed at 48 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab188569 Anti-IKK gamma/NEMO antibody [EPR14660] was shown to specifically react with IKK gamma/NEMO in wild-type HEK-293T cells in western blot. The band observed in the knockout cell line ab266674 (knockout cell lysate ab257153) lane below 48kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and IKK gamma/NEMO knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab188569 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-IKK gamma/NEMO antibody [EPR14660] (<a href='/en-us/products/primary-antibodies/ikk-gamma-nemo-antibody-epr14660-ab188569'>ab188569</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
IKBKG knockout HEK-293T cell lysate at 20 µg
Predicted band size: 48 kDa
Observed band size: 48 kDa
false
- WB
Lab
Western blot - Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell lysate (AB257153)
Lane 1 : Wild-type HEK-293T cell lysate (20µg)
Lane 2 : IKBKG knockout HEK-293T cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab178872 observed at 48 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab178872 Anti-IKK gamma/NEMO antibody [EPR16629] was shown to specifically react with IKK gamma/NEMO in wild-type HEK-293T cells in western blot. The band observed in the knockout cell line ab266674 (knockout cell lysate ab257153) lane below 48kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and IKK gamma/NEMO knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab178872 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (<a href='/en-us/products/primary-antibodies/ikk-gamma-nemo-antibody-epr16629-ab178872'>ab178872</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
IKBKG knockout HEK-293T cell lysate at 20 µg
Predicted band size: 48 kDa
Observed band size: 48 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell lysate (AB257153)
Allele-2 : 1 bp insertion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell lysate (AB257153)
Allele-1 : 13 bp deletion in exon 2
Complementary Products
Cell Lines & Lysates
AB266674
Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell line
Advanced Validation
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- 0
Primary Antibodies
AB178872
Anti-IKK gamma/NEMO antibody [EPR16629]
RabMAb|Recombinant|KO Validated
![Western blot - Anti-IKK gamma/NEMO antibody [EPR16629] (AB178872)](https://content.abcam.com/products/images/ikk-gamma-nemo-antibody-epr16629-ab178872--western-blot-img108169.jpg)
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Reactivity data
Product details
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com