Human IL6ST (CD130/gp130) knockout A549 cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
IL6ST KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and 4 bp deletion in exon4.
View Alternative Names
CD130, CD130 antigen, CDw130, GP130 RAPS, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL6 ST, IL6RB_HUMAN, Interleukin receptor beta chain, Interleukin-6 receptor subunit beta, Interleukin-6 signal transducer, Membrane glycoprotein 130, Membrane glycoprotein gp130, Oncostatin M receptor, Oncostatin M receptor alpha subunit, Oncostatin-M receptor subunit alpha, gp130
- WB
Lab
Western blot - Human IL6ST (CD130/gp130) knockout A549 cell lysate (AB257207)
Lane 1 : Wild-type A549 cell lysate (20 μg)
Lane 2 : IL6ST knockout A549 cell lysate (20 μg)
Lanes 1-2 : Merged signal (red and green). Green - ab217671 observed at 130 kDa. Red - loading control ab7291 observed at 50 kDa.
ab217671 Anti-CD130 (gp130) antibody [EPR21732] was shown to specifically react with CD130 (gp130) in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266938 (knockout cell lysate ab257207) was used. Wild-type and CD130 (gp130) knockout samples were subjected to SDS-PAGE. ab217671 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-CD130 (gp130) antibody [EPR21732] (<a href='/en-us/products/primary-antibodies/cd130-gp130-antibody-epr21732-ab217671'>ab217671</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
IL6ST knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human IL6ST (CD130/gp130) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-il6st-cd130-gp130-knockout-a549-cell-line-ab266938'>ab266938</a>)
Predicted band size: 104 kDa
Observed band size: 130 kDa
false
- WB
Lab
Western blot - Human IL6ST (CD130/gp130) knockout A549 cell lysate (AB257207)
Lane 1 : Wild-type A549 (human lung carcinoma epithelial cell) whole cell lysate, 20 ug
Lane 2 : IL6ST (CD130/gp130) knockout A549 (human lung carcinoma epithelial cell) whole cell lysate, 20 ug
Lane 3 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate, 20 ug
Blocking and diluting buffer and concentration : 5% NFDM/TBST
ab283685 was shown to specifically react with IL6ST (CD130/gp130) in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266938 (knockout cell lysate ab257207) was used. Wild-type and IL6ST (CD130/gp130) knockout samples were subjected to SDS-PAGE. ab283685 and ab181602 (Rabbit anti-GAPDH loading control) were incubated for 1 hour at room temperature at 1/1000 dilution and 1/200, 000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/50, 000 dilution and 1/100, 000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time : 70 seconds
All lanes:
Western blot - Anti-CD130 (gp130) antibody [EPR24557-50] (<a href='/en-us/products/primary-antibodies/cd130-gp130-antibody-epr24557-50-ab283685'>ab283685</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2:
IL6ST (CD130/gp130) knockout A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3:
PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 104 kDa
Observed band size: 130 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human IL6ST (CD130/gp130) knockout A549 cell lysate (AB257207)
Allele-1 : 4 bp deletion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human IL6ST (CD130/gp130) knockout A549 cell lysate (AB257207)
Allele-2 : 1 bp insertion in exon4
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD130 functions as part of the high-affinity receptor complex for certain cytokines. It partners with other receptors such as CD126 to form a complex that initiates signaling pathways after ligand binding contributing to a range of immune responses. Its role is thorough across many cellular processes including differentiation proliferation and apoptosis. In addition to immune signaling CD130 is involved in several growth factor signaling pathways.
Pathways
CD130 serves a significant role in signal transduction particularly in the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway. It works in concert with related proteins like JAK1 and STAT3 to transmit signals from extracellular cytokines to the nucleus influencing gene expression. Additionally CD130 can also be found active in the MAPK/ERK pathway which is important for cellular response to growth signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Primary Antibodies
AB283685
Anti-CD130 (gp130) antibody [EPR24557-50]
20ul selling size|RabMAb|Recombinant|KO Validated
![Immunoprecipitation - Anti-CD130 (gp130) antibody [EPR24557-50] (AB283685)](https://content.abcam.com/products/images/cd130-gp130-antibody-epr24557-50-ab283685--immunoprecipitation-img173734.jpg)
- 0
- 0
Primary Antibodies
AB271042
Anti-IL-6R antibody [EPR24322-143]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size
![Western blot - Anti-IL-6R antibody [EPR24322-143] (AB271042)](https://content.abcam.com/products/images/il-6r-antibody-epr24322-143-ab271042--western-blot-img7961.jpg)
- 5
- 1
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com