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AB257205

Human IL6ST (CD130/gp130) knockout HeLa cell lysate

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IL6ST KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and Insertion of the selection cassette in exon4.

View Alternative Names

CD130, CD130 antigen, CDw130, GP130 RAPS, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL6 ST, IL6RB_HUMAN, Interleukin receptor beta chain, Interleukin-6 receptor subunit beta, Interleukin-6 signal transducer, Membrane glycoprotein 130, Membrane glycoprotein gp130, Oncostatin M receptor, Oncostatin M receptor alpha subunit, Oncostatin-M receptor subunit alpha, gp130

3 Images
Western blot - Human IL6ST (CD130/gp130) knockout HeLa cell lysate (AB257205)
  • WB

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Western blot - Human IL6ST (CD130/gp130) knockout HeLa cell lysate (AB257205)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : IL6ST knockout HeLa cell lysate (20 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab217671 observed at 130 kDa. Red - loading control ab8245 observed at 37 kDa.

ab217671 Anti-CD130 (gp130) antibody [EPR21732] was shown to specifically react with CD130 (gp130) in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265767 (knockout cell lysate ab257205) was used. Wild-type and CD130 (gp130) knockout samples were subjected to SDS-PAGE. ab217671 and Anti-GAPDH antibody [6C5] - Loading Control were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD130 (gp130) antibody [EPR21732] (<a href='/en-us/products/primary-antibodies/cd130-gp130-antibody-epr21732-ab217671'>ab217671</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

IL6ST knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human IL6ST (CD130/gp130) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-il6st-cd130-gp130-knockout-hela-cell-line-ab265767'>ab265767</a>)

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 104 kDa

Observed band size: 130 kDa

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Sanger Sequencing - Human IL6ST (CD130/gp130) knockout HeLa cell lysate (AB257205)
  • Sanger seq

Unknown

Sanger Sequencing - Human IL6ST (CD130/gp130) knockout HeLa cell lysate (AB257205)

Allele-2 : Insertion of the selection cassette in exon4

Sanger Sequencing - Human IL6ST (CD130/gp130) knockout HeLa cell lysate (AB257205)
  • Sanger seq

Unknown

Sanger Sequencing - Human IL6ST (CD130/gp130) knockout HeLa cell lysate (AB257205)

Allele-1 : 1 bp insertion in exon4

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and Insertion of the selection cassette in exon4.

Disease

Adenocarcinoma

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
IL6ST
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD130 also known as gp130 or the IL-6 signal transducer (IL6ST) is a protein weighing around 130 kDa. This receptor is encoded by the IL6ST gene and widely expressed on the surface of cells across multiple tissues. CD130 functions mechanically as a signal transducing receptor facilitating the assembly of receptor complexes necessary for the initiation of signal transduction. It plays a major role in cytokine receptor signaling particularly interacting with interleukin-6 (IL-6) family cytokines.
Biological function summary

CD130 functions as part of the high-affinity receptor complex for certain cytokines. It partners with other receptors such as CD126 to form a complex that initiates signaling pathways after ligand binding contributing to a range of immune responses. Its role is thorough across many cellular processes including differentiation proliferation and apoptosis. In addition to immune signaling CD130 is involved in several growth factor signaling pathways.

Pathways

CD130 serves a significant role in signal transduction particularly in the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway. It works in concert with related proteins like JAK1 and STAT3 to transmit signals from extracellular cytokines to the nucleus influencing gene expression. Additionally CD130 can also be found active in the MAPK/ERK pathway which is important for cellular response to growth signals.

CD130 plays a connection especially to inflammatory conditions and certain cancers. Aberrant signaling involving CD130 has been linked to disorders like rheumatoid arthritis where it contributes to the inflammation process. Additionally it relates to multiple myeloma a cancer where abnormal CD130 interactions with proteins like IL-6 may enhance cell survival and proliferation presenting potential targets for therapeutic intervention.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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