Human ILKAP knockout HEK-293T cell lysate
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ILKAP KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2.
View Alternative Names
DKFZp434J2031, FLJ10181, ILKAP2, ILKAP3, ILKAP_HUMAN, Integrin linked kinase associated phosphatase, Integrin linked kinase associated serine/threonine phosphatase, Integrin-linked kinase-associated serine/threonine phosphatase 2C, MGC4846, PP2C-delta, Protein phosphatase 2c delta isozyme
- WB
Lab
Western blot - Human ILKAP knockout HEK-293T cell lysate (AB258000)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : ILKAP knockout HEK293T cell lysate (20 ug)
Lane 3 : HeLa cell lysate (20 ug)
Lane 4 : HAP1 cell lysate (20 ug)
ab196013 was shown to specifically react with ILKAP in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266475 (knockout cell lysate ab258000) was used. Wild-type and ILKAP knockout samples were subjected to SDS-PAGE. ab196013 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-ILKAP antibody [EPR16145] (<a href='/en-us/products/primary-antibodies/ilkap-antibody-epr16145-ab196013'>ab196013</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
ILKAP knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human ILKAP knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ilkap-knockout-hek-293t-cell-line-ab266475'>ab266475</a>)
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
HAP1 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 49 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human ILKAP knockout HEK-293T cell lysate (AB258000)
Homozygous : 5 bp deletion in exon 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The phosphatase activity of ILKAP plays a role in cellular adhesion and movement. ILKAP participates in cellular processes by associating with the ILK complex which includes other key proteins like paxillin and parvin. By modulating the phosphorylation state of substrates within this complex ILKAP influences focal adhesion dynamics and cytoskeletal organization which are critical for maintaining cellular architecture and motility.
Pathways
ILKAP acts within the integrin signaling and Wnt signaling pathways. These pathways are important in regulating cell movement differentiation and survival. ILKAP interacts with proteins like β-catenin in the Wnt pathway influencing gene expression critical for cell fate determination. Additionally ILKAP works alongside ILK in the integrin signaling pathway to affect cellular responses like proliferation and migration.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB196013
Anti-ILKAP antibody [EPR16145]
RabMAb|Recombinant|KO Validated|Trial Size|Trial Size|Trial Size
![Western blot - Anti-ILKAP antibody [EPR16145] (AB196013)](https://content.abcam.com/products/images/ilkap-antibody-epr16145-ab196013--western-blot-img5042.jpg)
- 0
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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