Human INF2 knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human INF2 knockout HeLa cell lysate (AB258923)
Allele-1 : 29 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human INF2 knockout HeLa cell lysate (AB258923)
Allele-3 : Insertion of the selection cassette in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human INF2 knockout HeLa cell lysate (AB258923)
Allele-2 : 1 bp insertion in exon2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Inverted Formin 2 acts in multiple cellular processes like cell migration and division. It is a part of the formin family of proteins which are known to regulate actin filament polymerization. INF2 forms complexes with other proteins including mitochondria-associated membranes (MAMs). It connects the endoplasmic reticulum to mitochondria which supports cellular activities like calcium ion transfer and lipid biosynthesis.
Pathways
INF2 interacts with the Rho family GTPases in the regulation of actin cytoskeleton dynamics. It plays important roles in the RhoA pathway assisting the polymerization and depolymerization of actin filaments which impacts cell movement and morphology. INF2 interacts with diaphanous-related formins (DRFs) including mDia1 to support these cellular functions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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