Human IP6K2 knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
IP6K2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.
View Alternative Names
ATP 1D myo inositol hexakisphosphate phosphotransferase, IHPK 2, IP6K2_HUMAN, Inositol hexakisphosphate kinase 2, InsP6 kinase 2, KIAA0263, OTTHUMP00000164824, P(i)-uptake stimulator, Pi uptake stimulator, PiUS
- Sanger seq
Unknown
Sanger Sequencing - Human IP6K2 knockout HeLa cell lysate (AB258924)
Homozygous : 1 bp insertion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IP6K2 influences several cellular processes including apoptosis and cell cycle regulation. It operates as a part of protein complexes that mediate the synthesis of inositol pyrophosphates which are important secondary messengers in cells. Through these roles IP6K2 helps regulate physiological functions such as insulin signaling and energy balance. Its interactions with various binding partners also enable it to influence cell survival and growth parameters.
Pathways
IP6K2 is actively involved in the inositol phosphate metabolism and apoptosis pathways. Inositol phosphate metabolism involves a range of signaling molecules where IP6K2 contributes to the generation of key signaling lipids. It works in conjunction with other kinases such as IP6K1 and phosphatidylinositol 3-kinase (PI3K) facilitating complex cellular activities. Through these pathways IP6K2 modulates processes that are essential for maintaining cell viability and proper cellular responses to external stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com