JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB258005

Human IPO4 (Importin4/Imp4) knockout HEK-293T cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

IPO4 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon4 and 1 bp insertion in exon4.

View Alternative Names

IPO4, IMP4B, Imp4b, RanBP4, RANBP4, Ran-binding protein 4, Imp4, Importin-4b, Importin-4

3 Images
Western blot - Human IPO4 (Importin4/Imp4) knockout HEK-293T cell lysate (AB258005)
  • WB

Unknown

Western blot - Human IPO4 (Importin4/Imp4) knockout HEK-293T cell lysate (AB258005)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : IPO4knockout HEK293T cell lysate (20 ug)
Lane 3 : HepG2 cell lysate (20 ug)
Lane 4 : A549 cell lysate (20 ug)

ab181046 was shown to specifically react with Importin4/Imp4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266175 (knockout cell lysate ab258005) was used. Wild-type and Importin4/Imp4 knockout samples were subjected to SDS-PAGE. ab181046 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (<a href='/en-us/products/primary-antibodies/importin4-imp4-antibody-epr13660-ab181046'>ab181046</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

IPO4 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human IPO4 (Importin4/Imp4) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ipo4-importin4-imp4-knockout-hek-293t-cell-line-ab266175'>ab266175</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 119 kDa

Observed band size: 130 kDa

false

Sanger Sequencing - Human IPO4 (Importin4/Imp4) knockout HEK-293T cell lysate (AB258005)
  • Sanger seq

Unknown

Sanger Sequencing - Human IPO4 (Importin4/Imp4) knockout HEK-293T cell lysate (AB258005)

Allele-2 : 1 bp insertion in exon4

Sanger Sequencing - Human IPO4 (Importin4/Imp4) knockout HEK-293T cell lysate (AB258005)
  • Sanger seq

Unknown

Sanger Sequencing - Human IPO4 (Importin4/Imp4) knockout HEK-293T cell lysate (AB258005)

Allele-1 : 10 bp deletion in exon4

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon4 and 1 bp insertion in exon4.

style
left-column

Complementary Products

Primary Antibodies

AB181046

Anti-Importin4/Imp4 antibody [EPR13660]

RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Anti-Importin4/Imp4 antibody [EPR13660] (AB181046)

  • 0
  • 0
Proteins & Peptides

AB259412

Recombinant human VEGF 165A protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (AB259412)

  • 0
  • 0
Proteins & Peptides

AB180317

Recombinant Human POLR1C protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human POLR1C protein (His tag N-Terminus) (AB180317)

  • 0
  • 0
Proteins & Peptides

AB180278

Recombinant Human EXOSC8 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human EXOSC8 protein (His tag N-Terminus) (AB180278)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258005-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human IPO4 knockout HEK293T cell lysate", "number":"AB258005-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB258005-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
IPO4
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Importin4 (Imp4) also known as Ipo4 is a nuclear import receptor with an approximate mass of 130 kDa. This protein facilitates the transport of molecules such as ribosomal proteins and histones into the nucleus. Expression of Importin4 occurs in various tissues including the brain testis and liver. Importin4 plays a significant mechanical role by recognizing nuclear localization signals on cargo molecules and mediating their translocation across the nuclear envelope.
Biological function summary

Importin4 functions as part of the nuclear transport system a complex network essential for cellular operations. Importin4 interacts with the nuclear pore complex to enable import of specific protein cargo into the nucleus. By partnering with karyopherins and Ran-GTPase Importin4 participates in the active transport mechanism ensuring the correct localization of proteins that are critical to genomic regulation processes.

Pathways

Importin4 plays a role in the nuclear transport pathway and is associated with the ribosome biogenesis pathway. This protein works alongside proteins like Ran-GTPase enhancing the translocation of ribosomal components pivotal for cellular growth and protein synthesis. Importin4 also aids in the shuttling of histones which are essential to chromatin assembly and DNA transcription regulation.

Importin4 has connections to cancer and neurodegenerative diseases. Altered expression and mutations in Importin4 may disrupt cellular homeostasis and nuclear transport linking it to tumorigenesis. Additionally associations with proteins such as karyopherin alpha importers indicate its involvement in pathological states affecting the nervous system potentially contributing to conditions such as Alzheimer's disease. Understanding Importin4's interactions could provide insights into these complex diseases.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com