Human IPPK (IPK1) knockout HEK-293T cell lysate
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IPPK KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon9 and 29 bp deletion in exon9.
View Alternative Names
6)P5 2-kinase, 6-pentakisphosphate 2-kinase, C9orf12, INSP5K2, IP5K, IPK1, IPK1 homolog, IPPK_HUMAN, Inositol-1, Inositol-pentakisphosphate 2-kinase, Ins(1, Ins(1,3,4,5,6)P5 2 kinase, InsP5 2-kinase, bA476B13.1, bA476B13.1 (novel protein), inositol 1,3,4,5,6 pentakisphosphate 2 kinase
- Sanger seq
Unknown
Sanger Sequencing - Human IPPK (IPK1) knockout HEK-293T cell lysate (AB258925)
Allele-1 : 29 bp deletion in exon9
- Sanger seq
Unknown
Sanger Sequencing - Human IPPK (IPK1) knockout HEK-293T cell lysate (AB258925)
Allele-2 : 1 bp deletion in exon9
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IPPK contributes to the regulation of inositol phosphate metabolism. It does not act in isolation but as part of a network involving inositol polyphosphate kinases and phosphatases that help maintain cellular processes such as signal transduction and ion homeostasis. Although IPPK doesn't form part of a larger complex its activity impacts various cell signaling pathways by altering the levels of its substrate and product.
Pathways
IPPK participates significantly in the phosphoinositide signaling pathway impacting processes such as cellular growth survival and apoptosis. This pathway also involves PI3Ks and PTEN which are critical in signal transduction. Moreover IPPK indirectly influences the synthesis of IP7 regulated by inositol hexakisphosphate kinases integrating its function into broader metabolic pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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