Human IRF2 knockout A549 cell lysate
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IRF2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon4 and 1 bp insertion in exon4.
View Alternative Names
DKFZp686F0244, IRF2_HUMAN, Interferon regulatory factor 2
- Sanger seq
Unknown
Sanger Sequencing - Human IRF2 knockout A549 cell lysate (AB258007)
Allele-1 : 14 bp deletion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human IRF2 knockout A549 cell lysate (AB258007)
Allele-2 : 1 bp insertion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IRF2 regulates immune responses by controlling the expression of interferon (IFN) responsive genes. It acts independently and as part of transcriptional complexes binding to specific DNA sequences within target genes. Its action prevents excessive activation of immune responses maintaining a balanced immune system. IRF2 also influences cell cycle regulation contributing to its role in cell growth and differentiation.
Pathways
And several cellular communication networks IRF2 is a central figure in the interferon signaling pathway interacting with other members of the IRF family like IRF1 and IRF3. These interactions help propagate signals necessary for effective immune responses. In conjunction with the JAK-STAT pathway IRF2 modulates expressions that control antiviral responses and cellular proliferation showing its critical placement in immune response regulation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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