Human ISM1 knockout A549 cell lysate
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ISM1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon3 and 1 bp deletion in exon3 and 7 bp deletion in exon3.
View Alternative Names
C20orf82, Chromosome 20 open reading frame 82, ISM, Isthmin, Isthmin 1, Isthmin 1 homolog (zebrafish), OTTHUMP00000030295, bA149I18.1, dJ1077I2.1, isthmin 1 angiogenesis inhibitor
- Sanger seq
Unknown
Sanger Sequencing - Human ISM1 knockout A549 cell lysate (AB258474)
Allele-1 : 1 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human ISM1 knockout A549 cell lysate (AB258474)
Allele-3 : 7 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human ISM1 knockout A549 cell lysate (AB258474)
Allele-2 : 10 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ISM1 functions in angiogenesis and is involved in controlling cell proliferation and apoptosis. It does not form part of a protein complex but interacts with other molecules to regulate these processes. In addition ISM1 can influence the modulation of immune responses and exhibits potential anti-inflammatory properties. This biological activity suggests that ISM1 is significant for tissue development and homeostasis.
Pathways
ISM1 integrates into the angiogenesis and apoptotic pathways. In the angiogenesis pathway it interacts with proteins like VEGFR2 which leads to the promotion of new blood vessel formation. Furthermore in the context of apoptosis ISM1 can modulate pathways involving the protein Bcl-2 which plays an important role in cell death regulation. These interactions highlight ISM1's participation in vital biological pathways that maintain cellular function and viability.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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